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Type: Artigo
Title: Collagen Peptides Ameliorate Intestinal Epithelial Barrier Dysfunction In Immunostimulatory Caco-2 Cell Monolayers Via Enhancing Tight Junctions
Author: Chen
Qianru; Chen
Oliver; Martins
Isabela M.; Hou
Hu; Zhao
Xue; Blumberg
Jeffrey B.; Li
Abstract: Dysfunction of the intestinal barrier plays a key role in the pathogenesis of inflammatory bowel disease (IBD) and multiple organ failure. The effect of Alaska pollock skin-derived collagen and its 3 tryptic hydrolytic fractions, HCP (6 kDa retentate), MCP (3 kDa retentate) and LCP (3 kDa permeate) on TNF-a induced barrier dysfunction was investigated in Caco-2 cell monolayers. TNF-a induced barrier dysfunction was significantly attenuated by the collagen and its peptide fractions, especially LCP, compared to TNF-alpha treated controls (P < 0.05). Compared to a negative control, 24 h pre-incubation with 2 mg mL(-1) LCP significantly alleviated the TNF-alpha induced breakdown of the tight junction protein ZO-1 and occludin and inhibited MLC phosphorylation and MLCK expression. The activation of NF kappa B and Elk-1 was suppressed by LCP. Thus, collagen peptides may attenuate TNF-alpha induced barrier dysfunction of Caco-2 cells by inhibiting the NF kappa B and ERK1/ 2-mediated MLCK pathway with associated decreases in ZO-1 and occludin protein expression.
Subject: Light-chain Kinase
Tnf-alpha Modulation
Alaska Pollock Skin
Protein Expression
Editor: Royal Soc Chemistry
Rights: fechado
Identifier DOI: 10.1039/c6fo01347c
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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