Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/328572
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authoremailleticiafa@fmrp.usp.brpt_BR
dc.typeArtigopt_BR
dc.titleCats (fam64a) Abnormal Expression Reduces Clonogenicity Of Hematopoietic Cellsen
dc.contributor.authorBarbuttipt_BR
dc.contributor.authorIsabella; Xavier-Ferruciopt_BR
dc.contributor.authorJuliana M.; Machado-Netopt_BR
dc.contributor.authorJoao Agostinho; Riconpt_BR
dc.contributor.authorLauremilia; Trainapt_BR
dc.contributor.authorFabiola; Bohlanderpt_BR
dc.contributor.authorStefan K.; Olalla Saadpt_BR
dc.contributor.authorSara Teresinha; Archangelopt_BR
dc.contributor.authorLeticia Frohlichpt_BR
unicamp.authorArchangelo, Leticia Frohlich] State Univ Campinas UNICAMP, Hematol & Hemotherapy Ctr, Carlos Chagas 480, Campinas, SP, Brazilpt_BR
unicamp.author.external[Barbutti, Isabellapt_BR
unicamp.author.externalXavier-Ferrucio, Juliana M.pt_BR
unicamp.author.externalMachado-Neto, Joao Agostinhopt_BR
unicamp.author.externalRicon, Lauremiliapt_BR
unicamp.author.externalOlalla Saad, Sara Teresinhapt_BR
unicamp.author.external[Machado-Neto, Joao Agostinhopt_BR
unicamp.author.externalTraina, Fabiola] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Internal Med, Sao Paulo, Brazilpt_BR
unicamp.author.external[Bohlander, Stefan K.] Univ Auckland, Dept Mol Med & Pathol, Auckland, New Zealandpt_BR
unicamp.author.external[Archangelo, Leticia Frohlich] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Cellular & Mol Biol & Pathogen Bioagents, Sao Paulo, Brazilpt_BR
unicamp.author.external[Xavier-Ferrucio, Juliana M.] Yale Stem Cell Ctr, Dept Lab Med, New Haven, CT USApt_BR
dc.subjectCats (fam64a)en
dc.subjectProliferationen
dc.subjectClonogenicityen
dc.subjectCalm/af10en
dc.subjectLeukemogenesisen
dc.description.abstractThe CATS (FAM64A) protein interacts with CALM (PICALM) and the leukemic fusion protein CALM/AF10. CATS is highly expressed in leukemia, lymphoma and tumor cell lines and its protein levels strongly correlates with cellular proliferation in both malignant and normal cells. In order to obtain further insight into CATS function we performed an extensive analysis of CATS expression during differentiation of leukemia cell lines. While CATS expression decreased during erythroid, megakaryocytic and monocytic differentiation, a markedly increase was observed in the ATRA induced granulocytic differentiation. Lentivirus mediated silencing of CATS in U937 cell line resulted in somewhat reduced proliferation, altered Cell Cycle progression and lower migratory ability in vitro; however was not sufficient to inhibit tumor growth in xenotransplant model. Of note, CATS knockdown resulted in reduced clonogenicity of CATS-silenced cells and reduced expression of the self-renewal gene, GLI-1. Moreover, retroviral mediated overexpression of the murine Cats in primary bone marrow cells lead to decreased colony formation. Although our in vitro data suggests that CATS play a role in cellular processes important for tumorigenesis, such as Cell Cycle control and clonogenicity, these effects were not observed in vivo.en
dc.relation.ispartofOncotargetpt_BR
dc.publisherImpact Journals Llcpt_BR
dc.publisherAlbanypt_BR
dc.date.issued2016pt_BR
dc.date.monthofcirculationoutpt_BR
dc.identifier.citationOncotarget. Impact Journals Llc, v. 7, p. 68385 - 68396, 2016.pt_BR
dc.language.isoEnglishpt_BR
dc.description.volume7pt_BR
dc.description.issuenumber42pt_BR
dc.description.firstpage68385pt_BR
dc.description.lastpage68396pt_BR
dc.rightsabertopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1949-2553pt_BR
dc.identifier.wosidWOS:000387446800055pt_BR
dc.identifier.doi10.18632/oncotarget.11630pt_BR
dc.identifier.urlhttp://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=11724&pubmed-linkout=1pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [101624/2011-5]pt_BR
dc.description.sponsorshipCoordenacao de Aperfeicoamento Pessoal de nivel Superior (CAPES)pt_BR
dc.description.sponsorshipFundacao de Amparo a Pesquisa de Sao Paulo (FAPESP) [2011/18188-0, 2011/51959-0]pt_BR
dc.description.sponsorshipLeukaemia and Blood Cancer New Zealandpt_BR
dc.description.sponsorshipfamily of Marijanna Kumerichpt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.date.available2017-11-13T13:43:32Z-
dc.date.accessioned2017-11-13T13:43:32Z-
dc.description.provenanceMade available in DSpace on 2017-11-13T13:43:32Z (GMT). No. of bitstreams: 1 000387446800055.pdf: 5403659 bytes, checksum: ee762f34fe3939fc5cf8a2d8e1f9b1e9 (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328572-
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