Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/328379
Type: Artigo
Title: Enamel And Dentine Demineralization By A Combination Of Starch And Sucrose In A Biofilm - Caries Model
Title Alternative: Enamel and dentine demineralization by a combination of starch and sucrose in a biofilm - caries model
Author: Botelho, Juliana Nunes
Villegas-Salinas, Mario
Troncoso-Gajardo, Pia
Giacaman, Rodrigo Andres
Cury, Jaime Aparecido
Abstract: Sucrose is the most cariogenic dietary carbohydrate and starch is considered non-cariogenic for enamel and moderately cariogenic for dentine. However, the cariogenicity of the combination of starch and sucrose remains unclear. The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization. Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva. They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose). To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium. Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified. Dentine and enamel demineralization was assessed by determining the percentage of surface hardness loss. Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001) on either enamel or dentine than those exposed to each carbohydrate alone. The findings suggest that starch increases the cariogenic potential of sucrose.
Sucrose is the most cariogenic dietary carbohydrate and starch is considered non-cariogenic for enamel and moderately cariogenic for dentine. However, the cariogenicity of the combination of starch and sucrose remains unclear. The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel and dentine demineralization. Biofilms of S. mutans UA159 were grown on saliva-coated enamel and dentine slabs in culture medium containing 10% saliva. They were exposed (8 times/day) to one of the following treatments: 0.9% NaCl (negative control), 1% starch, 10% sucrose, or 1% starch and 10% sucrose (starch + sucrose). To simulate the effect of human salivary amylase on the starch metabolization, the biofilms were pretreated with saliva before each treatment and saliva was also added to the culture medium. Acidogenicity of the biofilm was estimated by evaluating (2 times/day) the culture medium pH. After 4 (dentine) or 5 (enamel) days of growth, biofilms (n = 9) were individually collected, and the biomass, viable microorganism count, and polysaccharide content were quantified. Dentine and enamel demineralization was assessed by determining the percentage of surface hardness loss. Biofilms exposed to starch + sucrose were more acidogenic and caused higher demineralization (p < 0.0001) on either enamel or dentine than those exposed to each carbohydrate alone. The findings suggest that starch increases the cariogenic potential of sucrose
Subject: Amylases
Biofilms
Dental Caries
Dietary Carbohydrates
Tooth Demineralization
Biofilme
Carboidratos da dieta
Desmineralização do dente
Cárie dentária
Country: Brasil
Editor: Sociedade Brasileira de Pesquisa Odontológica
Citation: Brazilian Oral Research. Sociedade Brasileira De Pesquisa Odontologica, v. 30, p. , 2016.
Rights: aberto
Identifier DOI: 10.1590/1807-3107BOR-2016.vol30.0052
Address: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100250&lng=en&tlng=en
Date Issue: 2016
Appears in Collections:FOP - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000378324000046.pdf473.03 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.