Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/328119
Type: Artigo
Title: In Vitro Determination Of Extracellular Proteins From Xylella Fastidiosa
Author: Mendes
Juliano S.; Santiago
Andre S.; Toledo
Marcelo A. S.; Horta
Maria A. C.; de Souza
Alessandra A.; Tasic
Ljubica; de Souza
Anete P.
Abstract: The phytopathogen Xylella fastidiosa causes economic losses in important agricultural crops. Xylem vessel occlusion caused by biofilm formation is the major mechanism underlying the pathogenicity of distinct strains of X. fastidiosa. Here, we provide a detailed in vitro characterization of the extracellular proteins of X. fastidiosa. Based on the results, we performed a comparison with a strain J1a12, which cannot induce citrus variegated chlorosis symptoms when inoculated into citrus plants. We then extend this approach to analyze the extracellular proteins of X. fastidiosa in media supplemented with calcium. We verified increases in extracellular proteins concomitant with the days of growth and, consequently, biofilm development (330 days). Outer membrane vesicles carrying toxins were identified beginning at 10 days of growth in the 9a5c strain. In addition, a decrease in extracellular proteins in media supplemented with calcium was observed in both strains. Using mass spectrometry, 71 different proteins were identified during 30 days of X. fastidiosa biofilm development, including proteases, quorum-sensing proteins, biofilm formation proteins, hypothetical proteins, phage-related proteins, chaperones, toxins, antitoxins, and extracellular vesicle membrane components.
Subject: Xylella Fastidiosa
Extracellular Proteins
Biofilm
Calcium
Lc-ms/ms
Pw Medium
Editor: Frontiers Media SA
Lausanne
Citation: Frontiers In Microbiology. Frontiers Media Sa, v. 7, p. , 2016.
Rights: aberto
Identifier DOI: 10.3389/fmicb.2016.02090
Address: http://journal.frontiersin.org/article/10.3389/fmicb.2016.02090/full
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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