Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/328118
Type: Artigo
Title: Subtelomeric I-scel-mediated Double-strand Breaks Are Repaired By Homologous Recombination In Trypanosoma Cruzi
Author: Chiurillo
Miguel A.; Moraes Barros
Roberto R.; Souza
Renata T.; Marini
Marjorie M.; Antonio
Cristiane R.; Cortez
Danielle R.; Curto
Maria A.; Lorenz
Hernan A.; Schijman
Alejandro G.; Ramirez
Jose L.; da Silveira
Jose F.
Abstract: Trypanosoma cruzi chromosome ends are enriched in surface protein genes and pseudogenes (e.g., trans-sialidases) surrounded by repetitive sequences. It has been proposed that the extensive sequence variability among members of these protein families could play a role in parasite infectivity and evasion of host immune response. In previous reports we showed evidence suggesting that sequences located in these regions are subjected to recombination. To support this hypothesis we introduced a double-strand break (DSB) at a specific target site in a I cruzi subtelomeric region cloned into an artificial chromosome (pTAC). This construct was used to transfect T. cruzi epimastigotes expressing the I-Scel meganuclease. Examination of the repaired sequences showed that DNA repair occurred only through homologous recombination (HR) with endogenous subtelomeric sequences. Our findings suggest that DSBs in subtelomeric repetitive sequences followed by HR between them may contribute to increased variability in T. cruzi multigene families.
Subject: T. Cruzi
Telomere
I-scel Meganuclease
Double-strand Break
Homologous Recombination
Dna Repair
Artificial Chromosomes
Editor: Frontiers Media SA
Lausanne
Rights: aberto
Identifier DOI: 10.3389/fmicb.2016.02041
Address: http://journal.frontiersin.org/article/10.3389/fmicb.2016.02041/full
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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