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dc.titleCo-cultivation Of Aspergillus Nidulans Recombinant Strains Produces An Enzymatic Cocktail As Alternative To Alkaline Sugarcane Bagasse Pretreatmenten
dc.contributor.authorMatheus S.; Damasiopt_BR
dc.contributor.authorAndre R. de L.; Crnkovicpt_BR
dc.contributor.authorPaula M.; Pintopt_BR
dc.contributor.authorMarcelo R.; da Silvapt_BR
dc.contributor.authorAna M.; da Silvapt_BR
dc.contributor.authorJeanc. R.; Segatopt_BR
dc.contributor.authorFernando; de Lucaspt_BR
dc.contributor.authorRosymar C.; Jorgept_BR
dc.contributor.authorJoao A.; Polizelipt_BR
dc.contributor.authorMaria de L. T. de M.pt_BR[Damasio, Andre R. de L.] Univ Estadual Campinas, Inst Biol, Dept Biochem & Tissue Biol, Sao Paulo, Brazilpt_BR[Lima, Matheus S.pt_BR Lucas, Rosymar C.pt_BR, Joao A.pt_BR, Maria de L. T. de M.] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Biol, Sao Paulo, Brazilpt_BR[Crnkovic, Paula M.] Univ Sao Paulo, Dept Mech Engn, Sao Paulo, Brazilpt_BR[Pinto, Marcelo R.] Univ Uberaba, Lab Biopathol & Mol Biol, Uberaba, Brazilpt_BR[da Silva, Ana M.] Univ Fed Campina Grande, Cuite, Brazilpt_BR[da Silva, Jeanc. R.] Fed Inst Educ Sci & Technol Sao Paulo, Sao Paulo, Brazilpt_BR[Segato, Fernando] Univ Sao Paulo, Dept Biotechnol, Engn Sch Lorena, Sao Paulo, Brazilpt_BR[de Lucas, Rosymar C.] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, Sao Paulo, Brazilpt_BR
dc.subjectAspergillus Nidulansen
dc.subjectThermogravimetric Analysisen
dc.subjectDifferential Thermal Analysisen
dc.subjectNaoh Pretreatmenten
dc.subjectEnzymatic Pretreatmenten
dc.subjectSugarcane Bagasseen
dc.subjectEnzymatic Cocktailen
dc.description.abstractPlant materials represent a strategic energy source because they can give rise to sustainable biofuels through the fermentation of their carbohydrates. A clear example of a plant-derived biofuel resource is the sugar cane bagasse exhibiting 60-80% of fermentable sugars in its composition. However, the current methods of plant bioconversion employ severe and harmful chemical/physical pretreatments raising biofuel cost production and environmental degradation. Replacing these methods with co-cultivated enzymatic cocktails is an alternative. Here we propose a pretreatment for sugarcane bagasse using a multi-enzymatic cocktail from the co-cultivation of four Aspergillus nidulans recombinant strains. The co-cultivation resulted in the simultaneous production of GH51 arabinofuranosidase (AbfA), GH11 endo-1,4-xylanase (XInA), GH43 endo-1,5-arabinanase (AbnA) and GH12 xyloglucan specific endo-beta-1,4-glucanase (XegA). This core set of recombinant enzymes was more efficient than the alternative alkaline method in maintaining the cellulose integrity and exposing this cellulose to the following saccharification process. Thermogravimetric and differential thermal analysis revealed residual byproducts on the alkali pretreated biomass, which were not found in the enzymatic pretreatment. Therefore, the enzymatic pretreatment was residue-free and seemed to be more efficient than the applied alkaline method, which makes it suitable for bioethanol production.en
dc.relation.ispartofFrontiers in Microbiologypt_BR
dc.publisherFrontiers Media SApt_BR
dc.identifier.citationFrontiers In Microbiology. Frontiers Media Sa, v. 7, p. , 2016.pt_BR
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho de Desenvolvimento Cientilico e Tecnologico (CNPq)pt_BR
dc.description.sponsorshipNational System for Research on Biodiversity (Sisbiota-Brazil, CNPq) [563260/2010-6]pt_BR
dc.description.sponsorshipNational System for Research on Biodiversity (Sisbiota-Brazil, FAPESP) [2010/52322-3]pt_BR
dc.description.sponsorshipFAPESP [2012/20549-4, 2013/18910-3]pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.provenanceMade available in DSpace on 2017-11-13T13:23:30Z (GMT). No. of bitstreams: 1 000375385100001.pdf: 1396652 bytes, checksum: 6658498379c9e4b0b522fcb1a050aae6 (MD5) Previous issue date: 2016en
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