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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authoremailamaral.nanobiotech@gmail.compt_BR
dc.typeArtigopt_BR
dc.titleProduction Of Cyclodextrin Glycosyltransferase By Immobilized Bacillus Sp On Chitosan Matrixen
dc.contributor.authorEspt_BR
dc.contributor.authorIsmail; Ribeiropt_BR
dc.contributor.authorMaycon Carvalho; dos Santos Juniorpt_BR
dc.contributor.authorSamuel Rodrigues; Khaneghahpt_BR
dc.contributor.authorAmin Mousavi; Rodriguezpt_BR
dc.contributor.authorArmando Garcia; Amaralpt_BR
dc.contributor.authorAndre Correapt_BR
unicamp.author[Khaneghah, Amin Mousavi] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci, Sao Paulo, Brazilpt_BR
unicamp.author.external[Es, Ismailpt_BR
unicamp.author.externalRibeiro, Maycon Carvalho] Univ Fed Goias, Inst Biol Sci, Mol Biol & Genet, Goiania, Go, Brazilpt_BR
unicamp.author.external[dos Santos Junior, Samuel Rodriguespt_BR
unicamp.author.externalAmaral, Andre Correa] Univ Fed Goias, Inst Trop Pathol & Publ Hlth, Dept Biotechnol, Goiania, Go, Brazilpt_BR
unicamp.author.external[Rodriguez, Armando Garcia] Univ Fed Goias, Inst Biol Sci, Dept Biochem & Mol Biol, Goiania, Go, Brazilpt_BR
dc.subjectCgtaseen
dc.subjectCyclodextrinen
dc.subjectWhole-cell Immobilizationen
dc.subjectChitosanen
dc.subjectBacillusen
dc.description.abstractThe whole-cell immobilization on chitosan matrix was evaluated. Bacillus sp., as producer of CGTase, was grown in solid-state and batch cultivation using three types of starches (cassava, potato and cornstarch). Biomass growth and substrate consumption were assessed by flow cytometry and modified phenol-sulfuric acid assays, respectively. Qualitative analysis of CGTase production was determined by colorless area formation on solid culture containing phenolphthalein. Scanning electron microscopy (SEM) analysis demonstrated that bacterial cells were immobilized on chitosan matrix efficiently. Free cells reached very high numbers during batch culture while immobilized cells maintained initial inoculum concentration. The maximum enzyme activity achieved by free cells was 58.15 U ml(-1) (36 h), 47.50 U ml(-1) (36 h) and 68.36 U ml(-1) (36 h) on cassava, potato and cornstarch, respectively. CGTase activities for immobilized cells were 82.15 U ml(-1) (18 h) on cassava, 79.17 U ml(-1) (12 h) on potato and 55.37 U ml(-1) (in 6 h and max 77.75 U ml(-1) in 36 h) on cornstarch. Application of immobilization technique increased CGTase activity significantly. The immobilized cells produced CGTase with higher activity in a shorter fermentation time comparing to free cells.en
dc.relation.ispartofBioprocess And Biosystems Engineeringpt_BR
dc.publisherSpringerpt_BR
dc.publisherNew Yorkpt_BR
dc.date.issued2016pt_BR
dc.date.monthofcirculationoutpt_BR
dc.identifier.citationBioprocess And Biosystems Engineering. Springer, v. 39, p. 1487 - 1500, 2016.pt_BR
dc.language.isoEnglishpt_BR
dc.description.volume39pt_BR
dc.description.issuenumber10pt_BR
dc.description.firstpage1487pt_BR
dc.description.lastpage1500pt_BR
dc.rightsfechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1615-7591pt_BR
dc.identifier.eissn1615-7605pt_BR
dc.identifier.wosidWOS:000382117800001pt_BR
dc.identifier.doi10.1007/s00449-016-1625-6pt_BR
dc.identifier.urlhttps://link-springer-com.ez88.periodicos.capes.gov.br/article/10.1007%2Fs00449-016-1625-6pt_BR
dc.description.sponsorshipNational Council of Brazil for Scientific and Technological Development (CNPq)pt_BR
dc.description.sponsorshipCoordination for the Improvement of Higher Education Personnel in Brazil (CAPES)pt_BR
dc.description.sponsorshipCNPq-TWAS [3240274290]pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.date.available2017-11-13T13:23:11Z-
dc.date.accessioned2017-11-13T13:23:11Z-
dc.description.provenanceMade available in DSpace on 2017-11-13T13:23:11Z (GMT). No. of bitstreams: 1 000382117800001.pdf: 1862337 bytes, checksum: ccfe1f6a0d2b035897c59636bc9f56b6 (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/328050-
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