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Type: Artigo
Title: Production Of Cyclodextrin Glycosyltransferase By Immobilized Bacillus Sp On Chitosan Matrix
Author: Es
Ismail; Ribeiro
Maycon Carvalho; dos Santos Junior
Samuel Rodrigues; Khaneghah
Amin Mousavi; Rodriguez
Armando Garcia; Amaral
Andre Correa
Abstract: The whole-cell immobilization on chitosan matrix was evaluated. Bacillus sp., as producer of CGTase, was grown in solid-state and batch cultivation using three types of starches (cassava, potato and cornstarch). Biomass growth and substrate consumption were assessed by flow cytometry and modified phenol-sulfuric acid assays, respectively. Qualitative analysis of CGTase production was determined by colorless area formation on solid culture containing phenolphthalein. Scanning electron microscopy (SEM) analysis demonstrated that bacterial cells were immobilized on chitosan matrix efficiently. Free cells reached very high numbers during batch culture while immobilized cells maintained initial inoculum concentration. The maximum enzyme activity achieved by free cells was 58.15 U ml(-1) (36 h), 47.50 U ml(-1) (36 h) and 68.36 U ml(-1) (36 h) on cassava, potato and cornstarch, respectively. CGTase activities for immobilized cells were 82.15 U ml(-1) (18 h) on cassava, 79.17 U ml(-1) (12 h) on potato and 55.37 U ml(-1) (in 6 h and max 77.75 U ml(-1) in 36 h) on cornstarch. Application of immobilization technique increased CGTase activity significantly. The immobilized cells produced CGTase with higher activity in a shorter fermentation time comparing to free cells.
Subject: Cgtase
Whole-cell Immobilization
Editor: Springer
New York
Citation: Bioprocess And Biosystems Engineering. Springer, v. 39, p. 1487 - 1500, 2016.
Rights: fechado
Identifier DOI: 10.1007/s00449-016-1625-6
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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