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Type: Artigo
Title: Singlet Oxygen Production By Combining Erythrosine And Halogen Light For Photodynamic Inactivation Of Streptococcus Mutans
Author: Fracalossi
Camila; Nagata
Juliana Yuri; Pellosi
Diogo Silva; Suga Terada
Raquel Sano; Hioka
Noboru; Baesso
Mauro Luciano; Sato
Francielle; Rosalen
Pedro Luiz; Caetano
Wilker; Fujimaki
Abstract: Photodynamic inactivation of microorganisms is based on a photosensitizing substance which, in the presence of light and molecular oxygen, produces singlet oxygen, a toxic agent to microorganisms and tumor cells. This study aimed to evaluate singlet oxygen quantum yield of erythrosine solutions illuminated with a halogen light source in comparison to a LED array (control), and the photo dynamic effect of erythrosine dye in association with the halogen light source on Streptococcus mutans. Methods: Singlet oxygen quantum yield of erythrosine solutions was quantified using uric acid as a chemical-probe in an aqueous solution. The in vitro effect of the photodynamic antimicrobial activity of erythrosine in association with the halogen photopolimerizing light on Streptococcus mutans (UA 159) was assessed during one minute. Bacterial cultures treated with erythrosine alone served as negative control. Results: Singlet oxygen with 24% and 2.8% degradation of uric acid in one minute and a quantum yield of 0.59 and 0.63 was obtained for the erythrosine samples illuminated with the halogen light and the LED array, respectively. The bacterial cultures with erythrosine illuminated with the halogen light presented a decreased number of CFU mL(-1) in comparison with the negative control, with minimal inhibitory concentrations between 0.312 and 0.156 mg mL(-1). Conclusions: The photodynamic response of erythrosine induced by the halogen light was capable of killing S. mutans. Clinical trials should be conducted to better ascertain the use of erythrosine in association with halogen light source for the treatment of dental caries. (C) 2016 Elsevier B.V. All rights reserved.
Subject: Photodynamic Therapy
Streptococcus Mutans
Singlet Oxygen
Editor: Elsevier Science BV
Rights: fechado
Identifier DOI: 10.1016/j.pdpdt.2016.06.011
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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