Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327995
Type: Artigo
Title: Role Of Central Opioid On The Antinociceptive Effect Of Sulfated Polysaccharide From The Red Seaweed Solieria Filiformis In Induced Temporomandibular Joint Pain
Role of central opioid on the antinociceptive effect of sulfated polysaccharide from the red seaweed solieria filiformis in induced temporomandibular joint pain
Author: Fernandes Araújo, Ianna Wivianne
Chaves, Hellíada Vasconcelos
Pachêco, José Mário
Val, Danielle Rocha
Vieira, Lorena Vasconcelos
Santos, Rodrigo
Freitas, Raul Sousa
Rivanor, Renata Line
Monteiro, Valdécio Silvano
Clemente-Napimoga, Juliana Trindade
Bezerra, Mirna Marques
Barros Benevides, Norma Maria
Abstract: This study aimed to investigate the effect of sulfated polysaccharide from red seaweed Solieria filiformis (Fraction F II) in the inflammatory hypernociception in the temporomandibular joint (TMJ) of rats. Male Wistar rats were pretreated (30 min) with a subcutaneous injection (s.c.) of vehicle or FII (0.03, 03 or 3.0 mg/kg) followed by intra-TMJ injection of 1.5% Formalin or 5-hydroxytryptamine (5-HT, 225 mu g/TMJ). In other set of experiments rats were pretreated (15 min) with an intrathecal injection of the non-selective opioid receptors Naloxone, or mu-opioid receptor antagonist CTOP, or delta-opioid receptor Naltridole hydrochloride, or K-opioid receptor antagonist Nor-Binaltorphimine (Nor-BNI) followed by injection of FII (s.c.). After 30 min, the animals were treated with an intra-TMJ injection of 1.5% formalin. After TMJ treatment, behavioral nociception response was evaluated for a 45-min observation period, animals were terminally anesthetized and periarticular tissue, trigeminal ganglion and subnucleus caudalis (SC) were collected plasma extravasation and ELISA analysis. Pretreatment with F II significantly reduced formalin-and serotonin-induced TMJ nociception, inhibit the plasma extravasation and inflammatory cytokines release induced by 1.5% formalin in the TMJ. Pretreatment with intrathecal injection of Naloxone, CTOP, Naltridole or Nor-BNI blocked the antinociceptive effect of F II in the 1.5% formalin-induced TMJ nociception. In addition, F II was able to significantly increase the (beta-endorphin release in the subnucleus caudalis. The results suggest that FII has a potential antinociceptive and anti-inflammatory effect in the TMJ mediated by activation of opioid receptors in the subnucleus caudalis and inhibition of the release of inflammatory mediators in the periarticular tissue. (C) 2017 Published by Elsevier B.V.
This study aimed to investigate the effect of sulfated polysaccharide from red seaweed Solieria filiformis (Fraction F II) in the inflammatory hypernociception in the temporomandibular joint (TMJ) of rats. Male Wistar rats were pretreated (30 min) with a subcutaneous injection (s.c.) of vehicle or FII (0.03, 03 or 3.0 mg/kg) followed by intra-TMJ injection of 1.5% Formalin or 5-hydroxytryptamine (5-HT, 225 mu g/TMJ). In other set of experiments rats were pretreated (15 min) with an intrathecal injection of the non-selective opioid receptors Naloxone, or mu-opioid receptor antagonist CTOP, or delta-opioid receptor Naltridole hydrochloride, or K-opioid receptor antagonist Nor-Binaltorphimine (Nor-BNI) followed by injection of FII (s.c.). After 30 min, the animals were treated with an intra-TMJ injection of 1.5% formalin. After TMJ treatment, behavioral nociception response was evaluated for a 45-min observation period, animals were terminally anesthetized and periarticular tissue, trigeminal ganglion and subnucleus caudalis (SC) were collected plasma extravasation and ELISA analysis. Pretreatment with F II significantly reduced formalin-and serotonin-induced TMJ nociception, inhibit the plasma extravasation and inflammatory cytokines release induced by 1.5% formalin in the TMJ. Pretreatment with intrathecal injection of Naloxone, CTOP, Naltridole or Nor-BNI blocked the antinociceptive effect of F II in the 1.5% formalin-induced TMJ nociception. In addition, F II was able to significantly increase the (beta-endorphin release in the subnucleus caudalis. The results suggest that FII has a potential antinociceptive and anti-inflammatory effect in the TMJ mediated by activation of opioid receptors in the subnucleus caudalis and inhibition of the release of inflammatory mediators in the periarticular tissue
Subject: Marine Algae
Sulfated Polysaccharide
Nociception
Inflammation
Opioid Receptors
Alga marinha
Nociceptividade
Inflamação
Country: Holanda
Editor: Elsevier
Citation: International Immunopharmacology. Elsevier Science Bv, v. 44, p. 160 - 167, 2017.
Rights: fechado
Identifier DOI: 10.1016/j.intimp.2017.01.005
Address: https://www.sciencedirect.com/science/article/pii/S1567576917300139?via%3Dihub
Date Issue: 2017
Appears in Collections:FOP - Artigos e Outros Documentos

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