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Type: Artigo
Title: Insights Into The Plant Polysaccharide Degradation Potential Of The Xylanolytic Yeast Pseudozyma Brasiliensis
Author: Kaupert Neto
Antonio Adalberto; Borin
Gustavo Pagotto; Goldman
Gustavo Henrique; de Lima Damasio
Andre Ricardo; de Castro Oliveira
Juliana Velasco
Abstract: In second-generation (2G) bioethanol production, plant cell-wall polysaccharides are broken down to release fermentable sugars. The enzymes of this process are classified as carbohydrate-active enzymes (CAZymes) and contribute substantially to the cost of biofuel production. A novel basidiomycete yeast species, Pseudozyma brasiliensis, was recently discovered. It produces an endo-beta-1,4-xylanase with a higher specific activity than other xylanases. This enzyme is essential for the hydrolysis of biomass-derived xylan and has an important role in 2G bioethanol production. In spite of the P. brasiliensis biotechnological potential, there is no information about how it breaks down polysaccharides. For the first time, we characterized the secretome of P. brasiliensis grown on different carbon sources (xylose, xylan, cellobiose and glucose) and also under starvation conditions. The growth and consumption of each carbohydrate and the activity of the CAZymes of culture supernatants were analyzed. The CAZymes found in its secretomes, validated by enzymatic assays, have the potential to hydrolyze xylan, mannan, cellobiose and other polysaccharides. The data show that this yeast is a potential source of hydrolases, which can be used for biomass saccharification.
Subject: Pseudozyma Brasiliensis
Second-generation Bioethanol
Xylanolytic Yeast
Editor: Oxford Univ Press
Citation: Fems Yeast Research. Oxford Univ Press, v. 16, p. , 2016.
Rights: fechado
Identifier DOI: 10.1093/femsyr/fov117
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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