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Type: Artigo
Title: Cytotoxic Effect Of Erythroxylum Daphnites Extract Is Associated With G(1) Cell Cycle Arrest And Apoptosis In Oral Squamous Cell Carcinoma
Author: Elias
Silvia T.; Macedo
Carolina C. S.; Simeoni
Luiz A.; Silveira
Damaris; Magalhaes
Perola O.; Lofrano-Porto
Adriana; Coletta
Ricardo D.; Neves
Francisco A. R.; Guerra
Eliete N. S.
Abstract: Plant-derived molecules showing antineoplastic effects have recently gained increased attention as potential adjuvants to traditional therapies for various cancers. Cerrado biome in Brazil contains high floral biodiversity, but knowledge about the potential therapeutic effects of compounds derived from that flora is still limited. The present study investigated the antineoplastic activity of Erythroxylum daphnites Mart., a Brazilian native plant from Cerrado biome, in the SCC-9 oral squamous cell carcinoma cell line. Cells were treated with various concentrations of hexane extract of Erythroxylum daphnites leaves (EDH) and assessed for cytotoxicity, proliferation, and apoptosis. Thin layer chromatography was conducted to characterize the substances present in EDH. Our results showed that EDH exerted anti-proliferative effects in SCC-9 cells by stabilizing the cell cycle at G(1) phase in association with reduced intracellular levels of cyclins D and E and increased level of p21. EDH also demonstrated pro-apoptotic properties, as shown by an increased expression of caspase-3. Triterpenes were the major constituents of EDH. Our findings demonstrated a cytotoxic effect of EDH against SCC-9 cells in vitro mediated by the restraint of cellular proliferation and induction of apoptosis. Taken together, these findings support EDH constituents as potential therapeutic adjuvants for oral cancer.
Subject: Apoptosis
Cell Cycle
Cerrado Biome
Erythroxylum Daphnites
Oral Squamous Cell Carcinoma
Editor: Taylor & Francis Inc
Citation: Cell Cycle. Taylor & Francis Inc, v. 15, p. 948 - 956, 2016.
Rights: fechado
Identifier DOI: 10.1080/15384101.2016.1151583
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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