Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327882
Type: Artigo
Title: Dendritic Cells Stimulated By Cationic Liposomes
Author: Vitor
Micaela Tamara; Bergami-Santos
Patricia Cruz; Piedade Cruz
Karen Steponavicius; Pinho
Mariana Pereira; Marzagao Barbuto
Jose Alexandre; De La Torre
Lucimara Gaziola
Abstract: Immunotherapy of cancer aims to harness the immune system to detect and destroy cancer cells. To induce an immune response against cancer, activated dendritic cells (DCs) must present tumor antigens to T lymphocytes of patients. However, cancer patients' DCs are frequently defective, therefore, they are prone to induce rather tolerance than immune responses. In this context, loading tumor antigens into DCs and, at the same time, activating these cells, is a tempting goal within the field. Thus, we investigated the effects of cationic liposomes on the DCs differentiation/maturation, evaluating their surface phenotype and ability to stimulate T lymphocytes proliferation in vitro. The cationic liposomes composed by egg phosphatidylcholine, 1,2-dioleoyl-3-trimethylammonium propane and 1,2-dioleoylphosphatidylethanolamine (50/25/25% molar) were prepared by the thin film method followed by extrusion (65 nm, polydispersity of 0.13) and by the dehydration rehydration method (95% of the population 107 nm, polydispersity of 0.52). The phenotypic analysis of dendritic cells and the analysis of T lymphocyte proliferation were performed by flow cytometry and showed that both cationic liposomes were incorporated and activated dendritic cells. Extruded liposomes were better incorporated and induced higher CD86. expression for dendritic cells than dehydrated rehydrated vesicles. Furthermore, dendritic cells which internalized extruded liposomes also provided stronger T lymphocyte stimulation. Thus, cationic liposomes with a smaller size and polydispersity seem to be better incorporated by dendritic cells. Hence, these cationic liposomes could be used as a potential tool in further cancer immunotherapy strategies and contribute to new strategies in immunotherapy.
Subject: Cationic Liposomes
Dehydrated-rehydrated Vesicles
Extruded Liposomes
Cancer Immunotherapy
Dendritic Cells
Editor: Amer Scientific Publishers
Valencia
Citation: Journal Of Nanoscience And Nanotechnology. Amer Scientific Publishers, v. 16, p. 270 - 279, 2016.
Rights: fechado
Identifier DOI: 10.1166/jnn.2016.10714
Address: http://www.ingentaconnect.com/content/asp/jnn/2016/00000016/00000001/art00025
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000369680400025.pdf568.32 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.