Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327871
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authoremailmonica@ifi.unicamp.brpt_BR
dc.typeArtigopt_BR
dc.titleNanowire Arrays As Cell Force Sensors To Investigate Adhesin-enhanced Holdfast Of Single Cell Bacteria And Biofilm Stabilityen
dc.contributor.authorSahoopt_BR
dc.contributor.authorPrasana K.; Janissenpt_BR
dc.contributor.authorRichard; Monteiropt_BR
dc.contributor.authorMoniellen P.; Cavallipt_BR
dc.contributor.authorAlessandro; Murillopt_BR
dc.contributor.authorDuber M.; Merfapt_BR
dc.contributor.authorMarcus V.; Cesarpt_BR
dc.contributor.authorCarlos L.; Carvalhopt_BR
dc.contributor.authorHernandes F.; de Souzapt_BR
dc.contributor.authorAlessandra A.; Bakkerspt_BR
dc.contributor.authorErik P. A. M.; Cottapt_BR
dc.contributor.authorMonica A.pt_BR
unicamp.authorCotta, Monica A.] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Dept Appl Phys, BR-13083859 Sao Paulo, Brazilpt_BR
unicamp.author[Cesar, Carlos L.] Univ Estadual Campinas, Inst Phys Gleb Wataghin, Quantum Elect Dept, BR-13083859 Sao Paulo, Brazilpt_BR
unicamp.author[Carvalho, Hernandes F.] Univ Estadual Campinas, Inst Biol, Struct & Funct Biol Dept, BR-13083865 Sao Paulo, Brazilpt_BR
unicamp.author.external[Sahoo, Prasana K.pt_BR
unicamp.author.externalJanissen, Richardpt_BR
unicamp.author.externalMonteiro, Moniellen P.pt_BR
unicamp.author.externalMurillo, Duber M.pt_BR
unicamp.author.external[Janissen, Richard] Delft Univ Technol, Kavli Inst Nanosci, NL-2629 HZ Delft, Netherlandspt_BR
unicamp.author.external[Cavalli, Alessandropt_BR
unicamp.author.externalBakkers, Erik P. A. M.] Eindhoven Univ Technol, Dept Appl Phys, NL-5600 MB Eindhoven, Netherlandspt_BR
unicamp.author.external[Merfa, Marcus V.pt_BR
unicamp.author.externalde Souza, Alessandra A.] Agron Inst Campinas, Citrus Ctr APTA Sylvio Moreira, BR-13490970 Sao Paulo, Brazilpt_BR
dc.subjectBacterial Biofilmen
dc.subjectNanowire Arraysen
dc.subjectForce Sensoren
dc.subjectAdhesinen
dc.subjectCell Adhesionen
dc.subjectXylella Fastidiosaen
dc.description.abstractSurface attachment of a planktonic bacteria, mediated by adhesins and extracellular polymeric substances (EPS), is a crucial step for biofilm formation. Some pathogens can modulate cell adhesiveness, impacting host colonization and virulence. A framework able to quantify cell-surface interaction forces and their dependence on chemical surface composition may unveil adhesiveness control mechanisms as new targets for intervention and disease control. Here we employed InP nanowire arrays to dissect factors involved in the early stage biofilm formation of the phytopathogen Xylella fastidiosa. Ex vivo experiments demonstrate single-cell adhesion forces up to 45 nN, depending on the cell orientation with respect to the surface. Larger adhesion forces occur at the cell poles; secreted EPS layers and filaments provide additional mechanical support. Significant adhesion force enhancements were observed for single cells anchoring a biofilm and particularly on XadA1 adhesincoated surfaces, evidencing molecular mechanisms developed by bacterial pathogens to create a stronger holdfast to specific host tissues.en
dc.relation.ispartofNano Letterspt_BR
dc.publisherAmer Chemical Socpt_BR
dc.publisherWashingtonpt_BR
dc.date.issued2016pt_BR
dc.date.monthofcirculationjulpt_BR
dc.identifier.citationNano Letters. Amer Chemical Soc, v. 16, p. 4656 - 4664, 2016.pt_BR
dc.language.isoEnglishpt_BR
dc.description.volume16pt_BR
dc.description.issuenumber7pt_BR
dc.description.firstpage4656pt_BR
dc.description.lastpage4664pt_BR
dc.rightsfechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn1530-6984pt_BR
dc.identifier.eissn1530-6992pt_BR
dc.identifier.wosidWOS:000379794200099pt_BR
dc.identifier.doi10.1021/acs.nanolett.6b01998pt_BR
dc.identifier.urlhttp://pubs-acs-org.ez88.periodicos.capes.gov.br/doi/abs/10.1021/acs.nanolett.6b01998pt_BR
dc.description.sponsorshipFAPESP (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil) [2010/51748-7, 2013/02300-1]pt_BR
dc.description.sponsorshipCNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Brazil) [479486/2012-3]pt_BR
dc.description.sponsorshipCAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brazil)pt_BR
dc.description.sponsorshipFAPESPpt_BR
dc.description.sponsorshipFAPESP [08/57906-3]pt_BR
dc.description.sponsorshipCNPq [573913/2008-0]pt_BR
dc.description.sponsorshipDutch Technology Foundation STW of The Netherlands Organisation for Scientific Research (NWO) [11826]pt_BR
dc.description.sponsorshipDutch Ministry of Economic Affairspt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.date.available2017-11-13T13:22:22Z-
dc.date.accessioned2017-11-13T13:22:22Z-
dc.description.provenanceMade available in DSpace on 2017-11-13T13:22:22Z (GMT). No. of bitstreams: 1 000379794200099.pdf: 4747333 bytes, checksum: cc301f5460a815736026541cc6cb834e (MD5) Previous issue date: 2016en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/327871-
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