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Type: Artigo
Title: Enzymatic Potential Of Heterotrophic Bacteria From A Neutral Copper Mine Drainage
Author: da Costa
Bruna Zucoloto; Rodrigues
Viviane Drumond; de Oliveira
Valeria Maia; Mariscal Ottoboni
Laura Maria; Marsaioli
Anita Jocelyne
Abstract: Copper mine drainages are restricted environments that have been overlooked as sources of new biocatalysts for bioremediation and organic syntheses. Therefore, this study aimed to determine the enzymatic activities (esterase, epoxide hydrolase and monooxygenase) of 56 heterotrophic bacteria isolated from a neutral copper mine drainage (Sossego Mine, Canaa dos Carajas, Brazil). Hydrolase and monooxygenase activities were detected in 75% and 20% of the evaluated bacteria, respectively. Bacterial strains with good oxidative performance were also evaluated for biotransformation of organic sulfides. Fourteen strains with good enzymatic activity were identified by 16S rRNA gene sequencing, revealing the presence of three genera: Bacillus, Pseudomonas and Stenotrophomonas. The bacterial strains B. megaterium (SO5-4 and SO6-2) and Pseudomonas sp. (SO5-9) efficiently oxidized three different organic sulfides to their corresponding sulfoxides. In conclusion, this study revealed that neutral copper mine drainages are a promising source of biocatalysts for ester hydrolysis and sulfide oxidation/bioremediation. Furthermore, this is a novel biotechnological overview of the heterotrophic bacteria from a copper mine drainage, and this report may support further microbiological monitoring of this type of mine environment. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
Subject: Copper Mine Drainage
High-throughput Screening
Editor: Soc Brasileira Microbiologia
São Paulo
Citation: Brazilian Journal Of Microbiology. Soc Brasileira Microbiologia, v. 47, p. 846 - 852, 2016.
Rights: aberto
Identifier DOI: 10.1016/j.bjm.2016.07.004
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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