Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327799
Type: Artigo
Title: Curli Fimbria: An Escherichia Coli Adhesin Associated With Human Cystitis
Author: Cordeiro
Melina Aparecida; Werle
Catierine Hirsch; Milanez
Guilherme Paier; Yano
Tomomasa
Abstract: Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerise chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its mutant (Delta csgA) were analyzed for their adhesion ability over HTB-9 (human bladder carcinoma), Vero (kidney cells of African green monkey) and HUVEC (human umbilical vein) cells in the presence of alpha-D-mannose. All the wild-type UPEC strains tested (100%) were able to adhere to all three cell types, while the UPEC-4 Delta csgA mutant lost its adherence to HTB-9 but continued to adhere to the HUVEC and Vero cells. The results suggest that curli fimbria has an important role in the adhesion processes associated with human UPEC-induced cystitis. (C) 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda.
Subject: Curli Fimbria
Adhesion
Escherichia Coli
Upec
Editor: Soc Brasileira Microbiologia
São Paulo
Citation: Brazilian Journal Of Microbiology. Soc Brasileira Microbiologia, v. 47, p. 414 - 416, 2016.
Rights: aberto
Identifier DOI: 10.1016/j.bjm.2016.01.024
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S1517838216000757?via%3Dihub
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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