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Type: Artigo
Title: Plesiomonas Shigelloides Exports A Lethal Cytotoxic-enterotoxin (lce) By Membrane Vesicles
Author: Ludovico
Marilucia Santos; Martins
Luciano Moura; Romero Bianco
Juares Ednaldo; Tardelli de Jesus Andrade
Celia Guadalupe; Falcon
Rosabel; Joazeiro
Paulo Pinto; Viccari Gatti
Maria Silvia; Yano
Abstract: Plesiomonas shigelloides isolated from water in Brazil was previously described as a hemorrhagic heat-labile cytotoxic-enterotoxin producer. We purified this toxin from culture supernatants using ion metallic affinity chromatography (IMAC) followed by molecular exclusion chromatography. The pure toxin presented molecular mass of 50 kDa and isoelectric point (pI) around 6.9 by 2D electrophoresis. When injected intravenously, the purified cytotoxic-enterotoxin induced also severe spasms followed by sudden death of mice. Hence, we entitled it as lethal cytotoxic-enterotoxin (LCE). The presence of membrane vesicles (MVs) on cell surfaces of P. shigelloides was observed by scan electron microscopy (SEM). From these MVs the LCE toxin was extracted and confirmed by biological and serological assays. These data suggest that P. shigelloides also exports this cytotoxic-enterotoxin by membrane vesicles, a different mechanism of delivering extra cellular virulence factors, so far not described in this bacterium. (C) 2016 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda. This is an open access article under the CC BY-NC-ND license.
Subject: Lethal Cytotoxic Enterotoxin (lce)
Membrane Vesicles (mvs)
Plesiomonas Shigelloides
Toxin Transportation
Virulence Factors
Editor: Elsevier Brazil
Rio de Janeiro
Rights: fechado
Identifier DOI: 10.1016/j.bjid.2016.08.014
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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