Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327780
Type: Artigo
Title: Cytotoxic Factor Secreted By Escherichia Coli Associated With Sepsis Facilitates Transcytosis Through Human Umbilical Vein Endothelial Cell Monolayers
Author: Soares Tibo
Luiz Henrique; Bertol
Jessica Wildgrube; Bernedo-Navarro
Robert Alvin; Yano
Tomomasa
Abstract: Culture supernatant of sepsis-associated Escherichia coli (SEPEC) isolated from patients with sepsis caused loss of intercellular junctions and elongation of human umbilical vein endothelial cells (HUVEC). The cytotoxic factor was purified from culture supernatant of SEPEC 15 (serogroup 0153) by liquid chromatography process. PAGE (polyacrylamide gel electrophoresis) showed that the purified SEPEC cytotoxic factor had a molecular mass of similar to 150 kDa and consisted of at least two subunits. At the concentration of 1 CD50 (40 mu g/mL) did facilitate transcytosis through the HUVEC cells monolayer of SEPEC 15 as much as E. coli K12 within 30 min without affecting cell viability. These results suggest that this cytotoxic factor, named as SPF (SEPEC's permeabilizing factor), may be an important SEPEC virulence factor that facilitates bacterial access to the bloodstream. (C) 2016 Published by Elsevier Editora Ltda.
Subject: Cytotoxin (spf)
Sepsis
Transcytosis
Transendothelial Electrical Resistance
Editor: Elsevier Brazil
Rio de Janeiro
Citation: Brazilian Journal Of Infectious Diseases. Elsevier Brazil, v. 20, p. 298 - 302, 2016.
Rights: fechado
Identifier DOI: 10.1016/j.bjid.2016.01.003
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S1413867016300356?via%3Dihub
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000376443900014.pdf1.06 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.