Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327730
Type: Artigo
Title: Eugenia Aurata And Eugenia Punicifolia Hbk Inhibit Inflammatory Response By Reducing Neutrophil Adhesion, Degranulation And Net Release
Author: Costa
Mirian Feliciano; Jesus
Tais Iara; Pereira Lopes
Bruno Rafael; Figueiredo Angolini
Celio Fernando; Montagnolli
Abner; Gomes
Lorraine de Paula; Pereira
Gabriela Sterle; Tasca Gois Ruiz
Ana Lucia; Carvalho
Joao Ernesto; Eberlin
Marcos Nogueira; dos Santos
Catarina; Toledo
Karina Alves
Abstract: Eugenia spp. are used in popular medicine in the treatment of pain, diabetes, intestinal disorders and cough. The aim of the work is to evaluate, ex vivo and in vivo, the anti-inflammatory activity of the hydroethanolic extracts of the leaves of Eugenia aurata (EA) and Eugenia punicifolia HBK (EP) upon neutrophils. Methods: Ex vivo, isolated human neutrophils were sensitized by Eugenia extracts (0.1-1000 mu g/mL) and stimulated by PMA. In these conditions, different neutrophil activities related to inflammatory process were measured: adhesion, degranulation and NET release. Neutrophil viability and tumor line cells were monitored. In vivo, neutrophil influx was evaluated by peritonitis model performed in mice pretreated with different concentrations of Eugenia extracts. Phytochemical profile was assessed by mass spectrometry. Results: Ex vivo, EA and EP (1000 mu g/mL) reduced cell adhesion and degranulation, respectively. NET release was inhibited by EA and EP. Anti-inflammatory activities occurred in the absence of cytotoxicity. In vivo, both EA as EP inhibited neutrophil migration. The phytochemical profile revealed that EA contains myricitrin, rutin, quinic acid and quercetin derivatives. EP presents gallic acid, quercetin derivatives, syringic acid, ellagic acid, monogalloyl-glucose, glycosyringic acid, mudanoside B, HHDP glucose isomer and digalloylglucose isomer. EA and EP inhibit neutrophil migration by different pathways. Conclusion: Different chemical compositions may explain the anti-inflammatory effects described herein for EA and EP. Both extracts inhibit NET release but only EA reduces cell adhesion whereas EP decreases elastase secretion. This work contributes to the elucidation of cellular mechanisms related to the anti-inflammatory activity for leaves of E. aurata and E. punicifolia HBK.
Subject: Inflammation
Neutrophils
Eugenia Aurata
Eugenia Punicifolia (hbk)
Adhesion
Elastase
Editor: Biomed Central Ltd
London
Rights: aberto
Identifier DOI: 10.1186/s12906-016-1375-7
Address: https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1375-7/open-peer-review
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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