Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327667
Type: Artigo
Title: Prediction Of The In Planta Phakopsora Pachyrhizi Secretome And Potential Effector Families
Author: de Carvalho
Mayra C. da C. G.; Nascimento
Leandro Costa; Darben
Luana M.; Polizel-Podanosqui
Adriana M.; Lopes-Caitar
Valeria S.; Qi
Mingsheng; Rocha
Carolina S.; Carazzolle
Marcelo Falsarella; Kuwahara
Marcia K.; Pereira
Goncalo A. G.; Abdelnoor
Ricardo V.; Whitham
Steven A.; Marcelino-Guimaraes
Francismar C.
Abstract: Asian soybean rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, can cause losses greater than 80%. Despite its economic importance, there is no soybean cultivar with durable ASR resistance. In addition, the P. pachyrhizi genome is not yet available. However, the availability of other rust genomes, as well as the development of sample enrichment strategies and bioinformatics tools, has improved our knowledge of the ASR secretome and its potential effectors. In this context, we used a combination of laser capture microdissection (LCM), RNAseq and a bioinformatics pipeline to identify a total of 36 350 P. pachyrhizi contigs expressed in planta and a predicted secretome of 851 proteins. Some of the predicted secreted proteins had characteristics of candidate effectors: small size, cysteine rich, do not contain PFAM domains (except those associated with pathogenicity) and strongly expressed in planta. A comparative analysis of the predicted secreted proteins present in Pucciniales species identified new members of soybean rust and new Pucciniales- or P. pachyrhizi-specific families (tribes). Members of some families were strongly up-regulated during early infection, starting with initial infection through haustorium formation. Effector candidates selected from two of these families were able to suppress immunity in transient assays, and were localized in the plant cytoplasm and nuclei. These experiments support our bioinformatics predictions and show that these families contain members that have functions consistent with P. pachyrhizi effectors.
Subject: Effectors
Eti Suppression
Lcm
P
Pachyrhizi
Secretome
Soybean Rust
Transcriptome
Editor: Wiley-Blackwell
Hoboken
Citation: Molecular Plant Pathlogy. Wiley-blackwell, v. 18, p. 363 - 377, 2017.
Rights: fechado
Identifier DOI: 10.1111/mpp.12405
Address: http://onlinelibrary-wiley-com.ez88.periodicos.capes.gov.br/doi/10.1111/mpp.12405/abstract;jsessionid=3BD2A1D41BFA99DC23A7FFF1097E721E.f04t01
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000395552000004.pdf10.35 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.