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Type: Artigo
Title: Evaluating The Anesthetization And Fixation Efficacy Of ''soft'' And ''hard'' Freshwater Benthic Meiofauna: What Is The Best Method For Specimen Preservation?
Author: Garraffoni
Andre R. S.; Alcantara
Fabricio C.; Checon
Helio H.
Abstract: Sampling freshwater biological diversity is a challenge when it comes down to techniques for meiofauna fixation and preservation because this polyphyletic group of taxa is highly diverse. The aim of this study is to test the performance of three anesthetics (CO2, MgCl2 and low temperature) and three fixatives (formaldehyde 4 %; buffered formaldehyde 4 and 70 % ethanol) in the preservation of ''soft'' (gastrotrichs and rotifers) and ''hard'' (tardigrades and copepods) freshwater benthic meiofaunal assemblages. Due to these different morphological structures, we expected that treatment performance would vary among taxa in the quality of specimen fixation. Results revealed that the meiofaunal abundances of samples sorted alive or after the treatments with a coupling of anesthetics and fixatives were not different. However, preservation of specimens varied substantially among ''soft'' and ''hard'' meiofauna and among treatments. The use of 4 % buffered formaldehyde is highly recommended for freshwater meiofauna, while unbuffered formaldehyde should be avoided. Studies that have ''soft'' meiofauna as target organisms are recommended to use some type of anesthetic, although it is necessary to use a specific one for each taxon as they respond in different ways to different anesthetics.
Subject: Sampling Methods
Editor: Springer Japan KK
Citation: Limnology. Springer Japan Kk, v. 18, p. 209 - 218, 2017.
Rights: fechado
Identifier DOI: 10.1007/s10201-016-0502-y
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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