Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327629
Type: Artigo
Title: Acute Low-intensity Cycling With Blood-flow Restriction Has No Effect On Metabolic Signaling In Human Skeletal Muscle Compared To Traditional Exercise
Author: Smiles
William J.; Conceicao
Miguel S.; Telles
Guilherme D.; Chacon-Mikahil
Mara P. T.; Cavaglieri
Claudia R.; Vechin
Felipe C.; Libardi
Cleiton A.; Hawley
John A.; Camera
Donny M.
Abstract: Autophagy is an intracellular degradative system sensitive to hypoxia and exercise-induced perturbations to cellular bioenergetics. We determined the effects of low-intensity endurance-based exercise performed with blood-flow restriction (BFR) on cell signaling adaptive responses regulating autophagy and substrate metabolism in human skeletal muscle. In a randomized cross-over design, nine young, healthy but physically inactive males completed three experimental trials separated by 1 week of recovery consisting of either a resistance exercise bout (REX: 4 x 10 leg press repetitions, 70% 1-RM), endurance exercise (END: 30 min cycling, 70% VO2peak), or low-intensity cycling with BFR (15 min, 40% VO2peak). A resting muscle biopsy was obtained from the vastus lateralis 2 weeks prior to the first exercise trial and 3 h after each exercise bout. END increased ULK1(Ser757) phosphorylation above rest and BFR (similar to 37 to 51%, P < 0.05). Following REX, there were significant elevations compared to rest (similar to 348%) and BFR (similar to 973%) for p38 gamma MAPK(Thr180/Tyr182) phosphorylation (P < 0.05). Parkin content was lower following BFR cycling compared to REX (similar to 20%, P < 0.05). There were no exercise-induced changes in select markers of autophagy following BFR. Genes implicated in substrate metabolism (HK2 and PDK4) were increased above rest (similar to 143 to 338%) and BFR cycling (similar to 212 to 517%) with END (P < 0.001). A single bout of low-intensity cycling with BFR is insufficient to induce intracellular "stress" responses (e.g., high rates of substrate turnover and local hypoxia) necessary to activate skeletal muscle autophagy signaling.
Subject: Blood-flow Restriction
Exercise
Autophagy
Mitophagy
Editor: Springer
New York
Citation: European Journal Of Applied Physiology. Springer, v. 117, p. 345 - 358, 2017.
Rights: fechado
Identifier DOI: 10.1007/s00421-016-3530-8
Address: https://link.springer.com/article/10.1007/s00421-016-3530-8
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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