Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327528
Type: Artigo
Title: Effect Of Hemicellulolytic Enzymes To Improve Sugarcane Bagasse Saccharification And Xylooligosaccharides Production
Effect of hemicellulolytic enzymes to improve sugarcane bagasse saccharification and xylooligosaccharides production
Author: Goldbeck, Rosana
Gonçalves, Thiago A.
Damásio, André R.L.
Brenelli, Lívia B.
Wolf, Lúcia D.
Paixão, Douglas A.A.
Rocha, George J.M.
Squina, Fabio M.
Abstract: Enzymatic hydrolysis of lignocellulosic biomass is limited by economic considerations arising from enzyme production costs and specific activities. The effect of six hemicellulases on raw sugarcane bagasse and two types of pretreated sugarcane bagasse was investigated using experimental designs. Our strategy was successful for developing more efficient and less expensive enzymatic mixture, and also revealed that hemicellulase mixtures with multiple activities could be less effective than expected. In this study, only two hemicelulases, the endo-1,4-xylanases (GH11) from Penicillium funiculosum (XynC11/CAC15487) and the feruloyl esterase (CE1) from Clostridium thermocellum (CtFAEIATCC27405), effectively broke down hemicellulose from pretreated sugarcane bagasse (up to 65%), along with the production of xylooligosaccharides (XOS). Our results also demonstrated that GH11 and CE1 can improve biomass saccharification by cellulases. Treatment with these two enzymes followed by a commercial cellulase cocktail (Accellerase (R) 1500) increased saccharifi cation of pretreated lignocellulose by 24%. Collectively, our data contributes to the rational design of more efficient and less expensive enzyme mixtures, targeting the viable production of bioethanol and other biorefinery products. (C) 2016 Elsevier B.V. All rights reserved.
Enzymatic hydrolysis of lignocellulosic biomass is limited by economic considerations arising from enzyme production costs and specific activities. The effect of six hemicellulases on raw sugarcane bagasse and two types of pretreated sugarcane bagasse was
Subject: Hemicelulose
Enzimas
Bagaço de cana
Xilooligossacarideos
Sacarificação
Country: Países Baixos
Editor: Elsevier
Citation: Journal Of Molecular Catalysis B: Enzymatic. Elsevier Science Bv, v. 131, p. 36 - 46, 2016.
Rights: fechado
fechado
Identifier DOI: 10.1016/j.molcatb.2016.05.013
Address: https://www.sciencedirect.com/science/article/pii/S138111771630090X
Date Issue: 2016
Appears in Collections:IB - Artigos e Outros Documentos
FEA - Artigos e Outros Documentos

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