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Type: Artigo
Title: Layer-by-layer Assembly Of Functionalized Reduced Graphene Oxide For Direct Electrochemistry And Glucose Detection
Author: Tavares Mascagni
Daniela Branco; Miyazaki
Celina Massumi; da Cruz
Nilson Cristino; de Moraes
Marli Leite; Riul
Jr.; Ferreira
Abstract: We report an electrochemical glucose biosensor made with layer-by-layer (LbL) films of functionalized reduced graphene oxide (rGO) and glucose oxidase (GOx). The LbL assembly using positively and negatively charged rGO multilayers represents a simple approach to develop enzymatic biosensors. The electron transport properties of graphene were combined with the specificity provided by the enzyme. rGO was obtained and functionalized using chemical methods, being positively charged with poly(diallyldimethylammonium chloride) to form GPDDA, and negatively charged with poly(styrene sulfonate) to form GPSS. Stable aqueous dispersions of GPDDA and GPSS are easily obtained, enabling the growth of LbL films on various solid supports. The use of graphene in the immobilization of GOx promoted Direct Electron Transfer, which was evaluated by Cyclic Volt-ammetry. Amperometric measurements indicated a detection limit of 13.4 mu mol.L-1 and sensitivity of 2.47 mu mmol(-1).L for glucose with the (GPDDA/GPSS)(1)/(GPDDA/GOx)(2) architecture, whose thickness was 19.80 +/- 0.28 nm, as determined by Surface Plasmon Resonance (SPR). The sensor may be useful for clinical analysis since glucose could be detected even in the presence of typical interfering agents and in real samples of a lactose-free milk and an electrolyte solution to prevent dehydration. (C) 2016 Elsevier B.V. All rights reserved.
Subject: Glucose Oxidase Biosensor
Direct Electron Transfer
Self-assembled Films
Functionalized Rgo
Editor: Elsevier Science BV
Rights: fechado
Identifier DOI: 10.1016/j.msec.2016.06.001
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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