Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327433
Type: Artigo
Title: Anti-inflammatory Therapies In Tramp Mice: Delay In Pca Progression
Author: Kido
Larissa Akemi; Montico
Fabio; Sauce
Rafael; Macedo
Aline Barbosa; Minatel
Elaine; Vendramini Costa
Debora Barbosa; de Carvalho
Joao Ernesto; Pilli
Ronaldo Aloise; Alves Cagnon
Valeria Helena
Abstract: The aim of this study was to characterize the structural and molecular biology as well as evaluate the immediate and late responses of prostatic cancer in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model after treatment with goniothalamin (GTN) and celecoxib. The treated mice received GTN (150 mg/kg, gavage) or celecoxib (10 mg/kg, gavage) from 8 to 12 weeks of age. They were killed at different ages: the immediate-response groups at 12 weeks and the late-response groups at 22 weeks. The ventral prostate was collected for light microscopy, immunohistochemistry, western blotting, TUNEL, and ELISA. Morphological analyses indicated that GTN treatment delayed the progression of prostatic adenocarcinoma, leading to a significant decrease of prostatic lesion frequency in both experimental period responses to this treatment, mainly high-grade prostatic intraepithelial neoplasia and well-differentiated adenocarcinoma. Also, the celecoxib treatment showed a particular decrease in the proliferative processes (PCNA) in both the experimental periods. Despite celecoxib diminishing the COX2 and IGFR1 levels, GTN presented higher action spectrum considering the decrease of a greater molecular number involved in the proliferative and inflammatory processes in prostatic cancer. Goniothalamin attenuated the pro-inflammatory response in TRAMP prostatic microenvironment, delaying prostate cancer (PCa) progression. Celecoxib treatment was efficient in the regulation of COX2 in the TRAMP mice, mainly in the advanced disease grade. Finally, we concluded that inflammatory process control in early grades of PCa was crucial for the downregulation of the signaling pathways involved in the proliferative processes in advanced cancer grades.
Subject: Inflammation
Prostate Cancer
Tramp
Anti-inflammatory Therapies
Goniothalamin
Editor: Bioscientifica Ltd
Bristol
Citation: Endocrine-related Cancer. Bioscientifica Ltd, v. 23, p. 235 - 250, 2016.
Rights: fechado
Identifier DOI: 10.1530/ERC-15-0540
Address: http://erc.endocrinology-journals.org/content/23/4/235.long
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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