Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327319
Type: Artigo
Title: Comparative Analysis Of Expression Profiling Of The Trypsin And Chymotrypsin Genes From Lepidoptera Species With Different Levels Of Sensitivity To Soybean Peptidase Inhibitors
Author: Souza
Thais P.; Dias
Renata O.; Castelhano
Elaine C.; Brandao
Marcelo M.; Moura
Daniel S.; Silva-Filho
Marcio C.
Abstract: Peptidase inhibitors (Pis) are essential proteins involved in plant resistance to herbivorous insects, yet many insect species are able to escape the negative effects of these molecules. We compared the effects of acute and chronic ingestion of soybean peptidase inhibitors (SPIs) on Spodoptera frugiperda and Diatraea saccharalis, two Lepidoptera species with different sensitivities to SPI ingestion. We analyzed the trypsin and chymotrypsin gene expression profiles in both species. Acute exposure of S. frugiperda to the inhibitors activated seven genes (SfChy5, SfChy9, SfChy19, SfChy22, SfTry6, SfTry8, and SfTry10), whereas chronic exposure activated 16 genes (SfChy2, SfChy4, SfChy5, SfChy8, SfChy9, SfChy11, SfChy12, SfChy15, SfChy17, SfChy21, SfChy22, SfTry6, SfTry8, SfTry9, SfTry10, and SfTry12). By contrast, the challenge of D. saccharalis with SPIs did not differentially induce the expression of trypsin- or chymotrypsin-encoding genes, with the exception of DsChy7. Bayesian phylogenetic analysis of S. frugiperda trypsin protein sequences revealed two gene clades: one composed of genes responsive to the SPIs and a second composed of the unresponsive genes. D. saccharalis trypsin proteins were clustered nearest to the S. frugiperda unresponsive genes. Overall, our findings support a hypothesized mechanism of resistance of Noctuidae moths to SPIs, involving gene number expansion of trypsin and chymotrypsin families and regulation of gene expression, which could also explain the variable susceptibility between S. frugiperda and D. saccharalis to these plant inhibitors. (C) 2016 Elsevier Inc. All rights reserved.
Subject: Diatraea Saccharalis
Plant Peptidase Inhibitor
Serine Peptidase
Spodoptera Frugiperda
Editor: Elsevier Science Inc
New York
Rights: fechado
Identifier DOI: 10.1016/j.cbpb.2016.02.007
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S1096495916300239?via%3Dihub
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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