Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/327188
Type: Artigo
Title: Integrated Microfluidic Devices For The Synthesis Of Nanoscale Liposomes And Lipoplexes
Author: Balbino
Tiago A.; Serafin
Juliana M.; Radaic
Allan; de Jesus
Marcelo B.; de la Torre
Lucimara G.
Abstract: In this work, pDNA/cationic liposome (CL) lipoplexes for gene delivery were prepared in one-step using multiple hydrodynamic flow-focusing regions. The microfluidic platform was designed with two distinct regions for the synthesis of liposomes and the subsequent assembly with pDNA, forming lipoplexes. The obtained lipoplexes exhibited appropriate physicochemical characteristics for gene therapy applications under varying conditions of flow rate-ratio (FRR), total volumetric flow rate (QT) and pDNA content (molar charge ratio, R). The CLs were able to condense and retain the pDNA in the vesicular structures with sizes ranging from 140 nm to 250 nm. In vitro transfection assays showed that the lipoplexes prepared in one step by the two-stage configuration achieved similar efficiencies as lipoplexes prepared by conventional bulk processes, in which each step comprises a series of manual operations. The integrated microfluidic platform generates lipoplexes with liposome formation combined in-line with lipoplex assembly, significantly reducing the number of steps usually required to form gene carrier systems. (C) 2017 Elsevier B.V. All rights reserved.
Subject: Microfluidics
Gene Delivery
Dna
Liposomes
Lipoplexes
Nanocarriers
Editor: Elsevier Science BV
Amsterdam
Rights: fechado
Identifier DOI: 10.1016/j.colsurfb.2017.01.030
Address: http://www.sciencedirect.com/science/article/pii/S0927776517300395
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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