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Type: Artigo
Title: Ultrastructural Aspects Of Mouse Nerve-muscle Preparation Exposed To Bothrops Jararacussu And Bothrops Bilineatus Venoms And Their Toxins Bthtx-i And Bbil-tx: Unknown Myotoxic Effects
Author: Melare
Rodolfo; Floriano
Rafael Stuani; Gracia
Marta; Rodrigues-Simioni
Lea; da Cruz-Hofling
Maria Alice; Rocha
Abstract: Bites by Bothrops snakes normally induce local pain, haemorrhage, oedema and myonecrosis. Mammalian isolated nerve-muscle preparations exposed to Bothrops venoms and their phospholipase A(2) toxins (PLA(2)) can exhibit a neurotoxic pattern as increase in frequency of miniature endplate potentials (MEPPs) as well as in amplitude of end-plate potentials (EPPs); neuromuscular facilitation followed by complete and irreversible blockade without morphological evidence for muscle damage. In this work, we analysed the ultrastructural damage induced by Bothrops jararacussu and Bothrops bilineatus venoms and their PLA(2) toxins (BthTX-I and Bbil-TX) in mouse isolated nerve-phrenic diaphragm preparations (PND). Under transmission electron microscopy (TEM), PND preparations previously exposed to B. jararacussu and B. bilineatus venoms and BthTX-I and Bbil-TX toxins showed hypercontracted and loosed myofilaments; unorganized sarcomeres; clusters of edematous sarcoplasmic reticulum and mitochondria; abnormal chromatin distribution or apoptotic-like nuclei. The principal affected organelles, mitochondria and sarcoplasmic reticulum, were those related to calcium buffering and, resulting in sarcomeres and myofilaments hypercontraction. Schwann cells were also damaged showing edematous axons and mitochondria as well as myelin sheath alteration. These ultrastructural changes caused by both of Bothrops venoms and toxins indicate that the neuromuscular blockade induced by them in vitro can also be associated with nerve and muscle degeneration.
Subject: Asp49-pla2
Bothrops Venoms
Transmission Electron Microscopy
Editor: Wiley-Blackwell
Rights: fechado
Identifier DOI: 10.1002/jemt.22748
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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