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Type: Artigo
Title: Homeopathic Medicine Cantharis Modulates Uropathogenic E. Coli (upec)-induced Cystitis In Susceptible Mice
Author: Coelho
Cideli de Paula; Motta
Priscilla Dias; Petrillo
Mariana; Iovine
Renata de Oliveira; Dalboni
Luciane Costa; Santana
Fabiana Rodrigues; Correia
Michelle Sanchez Freitas; Casarin
Renato Correa Viana; Carvalho
Vania Maria; Bonamin
Leoni Villano
Abstract: This is a random blinded placebo controlled murine experimental model to study the effects of Cantharis 6 CH, a homeopathic medicine, on E coil-induced cystitis. Methods: 24 adult susceptible female BALB/c mice were inoculated with E coli - UPEC 04:K -:H5 by a transurethral catheter. Cantharis 6cH or vehicle (placebo) was offered to mice by free access into the drinking water (1:100), during 24 h after infection. Spleen, bladder and kidneys were processed for quantitative histopathology after immunohistochemistry, using anti-CD3, CD79, MIF, NK and VEGF antibodies; the cytokines present in the bladder washing fluid were measured using a LUMINEX-Magpix KIT. Mann Whitney and Fisher exact test were used as statistical analysis. Results: Cantharis 6 CH increased IL12p40, IFN-gamma and decreased IL10 concentrations in the bladder fluid (p <=, 0.05); in the bladder mucosa, it increased the ratio between B and T lymphocytes (31%) and between B lymphocytes and MIF+ macrophages (57%, p <=. 0.05). In the pelvis, instead, it decreased the BiT cells ratio (41%, p <= 0.05) and increased the M1/M2 macrophage ratio (42%, p <= 5 0.05). No differences were seen in the kidney and spleen analysis. Conclusion: The inverted balance of inflammatory cells and cytokines in bladder and pelvis mucosa shows specific local immune modulation induced by Cantharis 6cH. (C) 2017 Elsevier Ltd. All rights reserved.
Subject: Escherichia Coli
Editor: Academic Press Ltd- Elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.cyto.2017.01.014
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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