Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326951
Type: Artigo
Title: The Biodiversity Of Aspergillus Section Flavi And Aflatoxins In The Brazilian Peanut Production Chain
Author: Martins
Ligia Manoel; Sant'Ana
Anderson S.; Pelegrinelli Fungaro
Maria Helena; Silva
Josue Jose; do Nascimento
Maristela da Silva; Frisvad
Jens Christian; Taniwaki
Marta Hiromi
Abstract: A total of 119 samples of peanut were collected throughout the peanut production chain in Sao Paulo State, Brazil. The peanut samples were directly plated for determination of percentages of infection and a polyphasic approach was used to identify Aspergillus section Flavi species. Further, the potential for aflatoxin production by the isolates was tested using the agar plug technique and the presence of aflatoxins in peanuts was assessed using an immunoaffinity column followed by quantification using HPLC with reverse phase column and fluorescence detection. The limit of detection and quantification were 0.05 and 0.17 mu g/kg for total aflatoxins, respectively. Four species of Aspergillus section Flavi were isolated: A. caelatus (11), A. flavus (515), A. parasiticus (17) and A. tamarii (13). All isolates of A. parasiticus were able to produce aflatoxin B and G whereas aflatoxin B was produced by 50% of A. flavus isolates. Aflatoxins were found in 12 samples at concentrations ranging from 0.3 to 100 mu g/kg. The data reported in this study add information on the occurrence and biodiversity of fungi in peanuts at several stages of the production chain. The occurrence of aflatoxins is also of major relevance for continuous monitoring and assessment of likely exposure of consumers to aflatoxins through consumption of peanuts. (C) 2017 Elsevier Ltd. All rights reserved.
Subject: Aspergillus Section Flavi
Peanut Chain
Aflatoxins
Editor: Elsevier Science BV
Amsterdam
Rights: fechado
Identifier DOI: 10.1016/j.foodres.2017.02.006
Address: http://www.sciencedirect.com/science/article/pii/S0963996917300583?via%3Dihub
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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