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Type: Artigo
Title: Functional And Genetic Characterization Of Hydrocarbon Biodegrader And Exopolymer-producing Clones From A Petroleum Reservoir Metagenomic Library
Author: Vasconcellos
Suzan P.; Sierra-Garcia
Isabel N.; Dellagnezze
Bruna M.; Vicentini
Renato; Midgley
David; Silva
Cynthia C.; Neto
Eugenio V. Santos; Volk
Herbert; Hendry
Philip; Oliveira
Valeria M.
Abstract: Microbial degradation of petroleum is a worldwide issue, which causes physico-chemical changes in its compounds, diminishing its commercial value. Biosurfactants are chemically diverse molecules that can be produced by several microorganisms and can enable microbial access to hydrocarbons. In order to investigate both microbial activities, function-driven screening assays for biosurfactant production and hydrocarbon biodegradation were carried out from a metagenomic fosmid library. It was constructed from the total DNA extracted from aerobic and anaerobic enrichments from a Brazilian biodegraded petroleum sample. A sum of 10 clones were selected in order to evaluate their ability to produce exopolymers (EPS) with emulsifying activity, as well as to characterize the gene sequences, harbored by the fosmid clones, through 454 pyrosequencing. Functional analyses confirmed the ability of some clones to produce surfactant compounds. Regarding hydrocarbon as microbial carbon sources, n-alkane (in mixture or not) and naphthalene were preferentially consumed as substrates. Analysis of sequence data set revealed the presence of genes related to xenobiotics biodegradation and carbohydrate metabolism. These data were corroborated by the results of hydrocarbon biodegradation and biosurfactant production detected in the evaluated clones.
Subject: Fosmid Clones
Heterologous Expression
Editor: Taylor & Francis Ltd
Rights: fechado
Identifier DOI: 10.1080/09593330.2016.1218940
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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