Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326819
Type: Artigo
Title: Autophagy Proteins Are Modulated In The Liver And Hypothalamus Of The Offspring Of Mice With Diet-induced Obesity
Author: Reginato
Andressa; de Fante
Thais; Portovedo
Mariana; da Costa
Natalia Ferreira; Payolla
Tanyara Baliani; Miyamotto
Josiane Erica; Simino
Lais Angelica; Ignacio-Souza
Leticia M.; Torsoni
Marcio A.; Torsoni
Adriana S.; Milanski
Marciane
Abstract: Nutritional excess during pregnancy and lactation has a negative impact on offspring phenotype. In adulthood, obesity and lipid overload represent factors that compromise autophagy, a process of lysosomal degradation. Despite knowledge of the impact of obesity on autophagy, changes in offspring of obese dams have yet to be investigated. In this study, we tested the hypothesis that maternal obesity induced by a high fat diet (HFD) modulates autophagy proteins in the hypothalamus and liver of the offspring of mice. At birth (d0), offspring of obese dams (HFD-O) showed an increase in p62 protein and a decrease in LC3-II, but only in the liver. After weaning (d18), the offspring of HFD-O animals showed impairment of autophagy markers in both tissues compared to control offspring (SC-O). Between day 18 and day 42, both groups received a control diet and we observed that the protein content of p62 remained increased in the livers of the HFD-O offspring. However, after 82 days, we did not find any modulation in offspring autophagy proteins. On the other hand, when the offspring of obese dams that received an HFD from day 42 until day 82 (OH-H) were compared with the offspring from the controls that only received an HFD in adulthood (OC-H), we saw impairment in autophagy proteins in both tissues. In conclusion, this study describes that HFD-O offspring showed early impairment of autophagy proteins. Although the molecular mechanisms have not been explored, it is possible that changes in autophagy markers could be associated with metabolic disturbances of offspring. (C) 2016 Elsevier Inc. All rights reserved.
Subject: High-fat Diet
Obesity
Offspring
Autophagy
Hypothalamus
Liver
Editor: Elsevier Science Inc
New York
Rights: fechado
Identifier DOI: 10.1016/j.jnutbio.2016.04.002
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0955286316300468?via%3Dihub
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000380421700004.pdf1.52 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.