Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326732
Type: Artigo
Title: Lipid Profiles Of Canine Spermatozoa As Revealed Via Matrix-assisted Laser Desorption/ionization Mass Spectrometry
Lipid profiles of canine spermatozoa as revealed via matrix‐assisted laser desorption/ionization mass spectrometry
Author: Braga Jr., LT
Bravo, NRS
Belaz, KRA
Zampieri, D
Eberlin, MN
Conforti, VA
Abstract: In this study, we investigated the ability of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to characterize the lipid contents of canine spermatozoa. For that, samples of pure semen were analysed. Indeed, quite comprehensive lipid coverage was observed, and the most abundant phospholipid ions detected were from four phosphatidylcholines, that is those of m/z 760.6; 782.6; 808.6; and 830.6 and one of m/z 725.6 from a sphingomyelin. In conclusion, MALDI-MS was found to offer an easy, fast, accurate, and sensitive analytical method for lipid profiling in canine spermatozoa and could be used as a tool to select sires by assessing the relationship between sperm lipid profiles and variables such as age and breeding history as well as to study the effects of cryopreservation on lipid contents.
In this study, we investigated the ability of matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) to characterize the lipid contents of canine spermatozoa. For that, samples of pure semen were analysed. Indeed, quite comprehensive lip
Subject: Perfil lipídico
Espermatozóides
Espectrometria de massas por ionização e dessorção a laser assistida por matriz
Country: Alemanha
Editor: Wiley
Citation: Reproduction In Domestic Animals. Wiley-blackwell, v. 51, p. 1055 - 1058, 2016.
Rights: fechado
Fechado
Identifier DOI: 10.1111/rda.12792
Address: https://onlinelibrary.wiley.com/doi/full/10.1111/rda.12792
Date Issue: 2016
Appears in Collections:IQ - Artigos e Outros Documentos

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