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Type: Artigo
Title: Early Developmental Stages Of Ascaris Lumbricoides Featured By High-resolution Mass Spectrometry
Author: Odir Rodrigues Melo
Carlos Fernando; Esteves
Cibele Zanardi; de Oliveira
Rosimeire Nunes; Guerreiro
Tatiane Melina; de Oliveira
Diogo Noin; Lima
Estela de Oliveira; Mine
Julio Cesar; Allegretti
Silmara Marques; Catharino
Rodrigo Ramos
Abstract: Ascaris lumbricoides is responsible for a highly disseminated helminth parasitic disease, ascariosis, a relevant parasitosis that responds for great financial burden on the public health system of developing countries. In this work, metabolic fingerprinting using high-resolution mass spectrometry (HRMS) was employed to identify marker molecules from A. lumbricoides in different development stages. We have identified nine biomarkers, such as pheromones and steroidal prohormones in early stages, among other molecules in late development stages, making up four molecules for fertilized eggs, four marker molecules for first larvae (L1) and one marker molecule for third larvae (L3). Therefore, our findings indicate that this approach is suitable for biochemical characterization of A. lumbricoides development stages. Moreover, the straightforward analytical method employed, with almost no sample preparation from a complex matrix (feces) using high-resolution mass spectrometry, suggests that it is possible to seek for an easier and faster way to study animal molding processes.
Subject: Metabolomics
Mass Spectrometry
Ascaris Lumbricoides
Editor: Springer
New York
Rights: fechado
Identifier DOI: 10.1007/s00436-016-5183-2
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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