Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326687
Type: Artigo
Title: Claudin-3 And Occludin Content In The Glands Of Colonic Mucosa Devoid From Fecal Stream Submitted To Topical Intervention With Oil Extract Of Curcuma Longa
Author: Real Martinez
Carlos Augusto; Kadri
Caled Jaoudat; Kanno
Danilo Toshio; Tiburcio Alves Junior
Antonio Jose; Rodrigues Coy
Claudio Saddy; Pereira
Jose Aires
Abstract: To evaluate the inflammatory intensity and measure the tissue content of the proteins claudin-3 and occludin in the colonic mucosa without fecal stream submit to intervention with curcumin. Methods: Thirty-six rats were submitted to a proximal colostomy and a distal mucous fistula and divided into two groups according to sacrifice to be performed two or four weeks. Each group was divided into three subgroups according daily application of enemas containing saline, curcumin at 50 mg/kg/day or 200 mg/kg/day. Colitis was diagnosed by histological analysis. Claudin-3 and occludin were determined by immunohistochemistry. The tissue content of claudin-3 and occludin were quantified by computer-assisted image analysis. MannWhitney, Student t and ANOVA tests were used to analyze the results establishing the level of significance of 5% for both (p< 0.05). Results: Curcumin at both concentrations reduces the inflammation and preserves the tissue content of the proteins claudin-3 and occludin, which was related to the concentration used and to the time of the intervention. Conclusion: The application of enemas with curcumin reduces inflammation and preserves the tissue content of the proteins claudin-3 and occludin in the colonic mucosa devoid from the fecal stream.
Subject: Colon
Colitis
Claudin-3
Occludin
Image Processing
Computer-assisted
Curcuma
Rats
Editor: Acta Cirurgica Brasileira
São Paulo
Rights: aberto
Identifier DOI: 10.1590/s0102-865020170108
Address: http://www.scielo.br/scielo.php?pid=S0102-86502017000100065&script=sci_arttext&tlng=pt
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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