Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326636
Type: Artigo
Title: Construction Of A Supercritical Fluid Extraction (sfe) Equipment: Validation Using Annatto And Fennel And Extract Analysis By Thin Layer Chromatography Coupled To Image
Author: Flores Johner
Julio Cezar; de Almeida Meireles
Maria Angela
Abstract: The present work describes setting up a laboratory unit for supercritical fluid extraction. In addition to its construction, a survey of cost was done to compare the cost of the homemade unit with that of commercial units. The equipment was validated using an extraction of annatto seeds' oil, and the extraction and fractionation of fennel oil were used to validate the two separators; for both systems, the solvent was carbon dioxide. The chemical profiles of annatto and fennel extracts were assessed using thin layer chromatography; the images of the chromatographic plates were processed using the free ImageJ software. The cost survey showed that the homemade equipment has a very low cost (similar to US$ 16,000) compared to commercial equipment. The extraction curves of annatto were similar to those obtained in the literature (yield of 3.8% oil). The separators were validated, producing both a 2.5% fraction of fennel seed extract rich in essential oils and another extract fraction composed mainly of oleoresins. The ImageJ software proved to be a low-cost tool for obtaining an initial evaluation of the chemical profile of the extracts.
Subject: Supercritical Fluid Extraction
Homemade Extraction Unit
Annatto
Fennel
Thin Layer Chromatography
Imagej Software
Editor: Soc Brasileira Ciencia Tecnologia Alimentos
Campinas
Rights: aberto
Identifier DOI: 10.1590/1678-457X.0027
Address: http://www.scielo.br/scielo.php?pid=S0101-20612016000200210&script=sci_arttext
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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