Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326634
Type: Artigo
Title: Duodenal-jejunal Bypass Normalizes Pancreatic Islet Proliferation Rate And Function But Not Hepatic Steatosis In Hypothalamic Obese Rats
Author: Cantelli
K. R.; Soares
G. M.; Ribeiro
R. A.; Balbo
S. L.; Lubaczeuski
C.; Boschero
A. C.; Araujo
A. C. F.; Bonfleur
M. L.
Abstract: Modifications in life-style and/or pharmacotherapies contribute to weight loss and ameliorate the metabolic profile of dietinduced obese humans and rodents. Since these strategies fail to treat hypothalamic obesity, we have assessed the possible mechanisms by which duodenal-jejunal bypass (DJB) surgery regulates hepatic lipid metabolism and the morphophysiology of pancreatic islets, in hypothalamic obese (HyO) rats. During the first 5 days of life, male Wistar rats received subcutaneous injections of monosodium glutamate (4 g/kg body weight, HyO group), or saline (CTL). At 90 days of age, HyO rats were randomly subjected to DJB (HyO DJB group) or sham surgery (HyO Sham group). HyO Sham rats were morbidly obese, insulin resistant, hypertriglyceridemic and displayed higher serum concentrations of non-esterified fatty acids (NEFA) and hepatic triglyceride (TG). These effects were associated with higher expressions of the lipogenic genes and fatty acid synthase (FASN) protein content in the liver. Furthermore, hepatic genes involved in beta-oxidation and TG export were down-regulated in HyO rats. In addition, these rats exhibited hyperinsulinemia, beta-cell hypersecretion, a higher percentage of islets and beta-cell area/pancreas section, and enhanced nuclear content of Ki67 protein in islet-cells. At 2 months after DJB surgery, serum concentrations of TG and NEFA, but not hepatic TG accumulation and gene and protein expressions, were normalized in HyO rats. Insulin release and Ki67 positive cells were also normalized in HyO DJB islets. In conclusion, DJB decreased islet-cell proliferation, normalized insulinemia, and ameliorated insulin sensitivity and plasma lipid profile, independently of changes in hepatic metabolism.
Subject: Duodenal-jejunal Bypass
Hepatic Fatty Acid Metabolism
Hypothalamic Obesity
Ki67
Insulin Secretion
Editor: Assoc Bras Divulg Cientifica
São Paulo
Rights: aberto
Identifier DOI: 10.1590/1414-431X20175858
Address: http://www.scielo.br/scielo.php?pid=S0100-879X2017000500601&script=sci_arttext
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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