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Type: Artigo
Title: On-line Solid Phase Extraction Coupled With Liquid Chromatography Tandem Mass Spectrometry For The Determination Of Codeine In Human Plasma
Author: Cruz
Alessandro de C.; Suenaga
Eunice M.; Abib
Eduardo; Pedrazzoli
Abstract: A specific LC-MS/MS method was developed and validated for automated determination of codeine in human plasma, using on-line solid phase extraction (SPE) system coupled with positive ion electrospray ionization tandem mass spectrometry. The method allowed plasma direct injection onto cartridge without sample pre-treatment. Total analysis time per run was 3 min, allowing high-throughput for codeine determination. SPE on-line along a monolithic column (Chromolith Performance RP-18e, 100 mm x 4.6 mm) demonstrated to be highly effective in terms of backpressure, separation speed and peak asymmetry. Calibration curves range was linear 5.0-200 ng mL(-1). Method showed an excellent intra-day and inter-day precision ranged from 2.34 to 7.25% (CV%) as well as great intra-day and inter-day accuracy, ranging from 97.64 to 110% (RE%). SPE-LC-MS/MS method provided selectivity, accuracy, precision, fastness and high-throughput to assess codeine pharmacokinetics in human plasma samples.
Subject: On-line Spe
Monolithic Column
Editor: Soc Brasileira Quimica
São Paulo
Citation: Quimica Nova. Soc Brasileira Quimica, v. 40, p. 25 - 29, 2017.
Rights: aberto
Identifier DOI: 10.21577/0100-4042.20160153
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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