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Type: Artigo
Title: Hydrophilic Interaction Chromatography (hilic): State Of The Art And Applications
Author: Azevedo da Silva
Carla Grazieli; Grespan Bottoli
Carla Beatriz; Collins
Carol H.
Abstract: Hydrophilic interaction liquid chromatography (HILIC) has been gaining increased attention for its effective separation of highly polar compounds, which include carbohydrates, amino acids, pharmaceutical compounds, proteins, glycoproteins, nucleosides, etc. Polar compounds are usually poorly retained on reverse-phase liquid chromatography (RP-HPLC) columns or have poor solubility in the apolar mobile phase of normal-phase high performance liquid chromatography (NP-HPLC). Since HILIC uses organic solvents such as ACN or MeOH (>70%), also used in RP-HPLC and polar stationary phases similar to NP-HPLC (bare silica, diol, amino, amide, saccharide, zwitterionic stationary phases, etc.), it represents a hybrid of the two separation modes. The high organic content in the MP leads to good compatibility with mass spectrometry (MS), increasing the detectivity. This review describes the fundamentals of HILIC and highlights some interesting applications.
Subject: Hydrophilic Interaction Chromatography
Separation Of Polar Compounds
Hilic Separations
High Performance Liquid Chromatography
Polar Stationary Phases
Editor: Soc Brasileira Quimica
São Paulo
Citation: Quimica Nova. Soc Brasileira Quimica, v. 39, p. 210 - 220, 2016.
Rights: aberto
Identifier DOI: 10.5935/0100-4042.20160003
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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