Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326569
Type: Artigo
Title: Hypoxia-driven Changes In Glycolytic And Tricarboxylic Acid Cycle Metabolites Of Two Nodulated Soybean Genotypes
Author: Borella
Junior; Oliveira
Halley Caixeta; Colares de Oliveira
Denise dos Santos; Bolacel Braga
Eugenia Jacira; Barneche de Oliveira
Ana Claudia; Sodek
Ladaslav; do Amarante
Luciano
Abstract: Oxygen deprivation triggers changes at different levels of carbon and nitrogen metabolism, which may differ between plant-genotypes. The aim of this study was to evaluate the hypoxia-induced alterations of carbon and nitrogen metabolites in relation to alanine aminotransferase (AlaAT; EC 2.6.1.2) activity in two genotypes of nodulated soybean (Glycine max). Nodulated soybean plants (Fundacep 53 RR and BRS Macota) were grown in vermiculite and transferred to a hydroponic system at the early reproductive stage. The root system was subjected to hypoxia by continuously flushing the solution with N-2 gas for 24 or 72 h. For recovery, after 72 h in hypoxia, plants returned to normoxic conditions after transfer to vermiculite for 24 and 72 h. Root and nodule organic acids and amino acids were analysed by gas chromatography-mass spectrometry and high-performance liquid chromatography, respectively. Relative expression of AlaAT and AIaAT activity were also verified in both genotypes. Plants of Fundacep and Macota genotypes responded distinctly to hypoxia. In root tissues, Fundacep presented higher pyruvate and lactate accumulation than Macota, indicating higher glycolytic and fermentation rates. Furthermore, Fundacep responded more effectively on recovery by restoring pre-hypoxic levels of the metabolites. Although the amino acid composition did not differ between the genotypes, there was a clear link between glycolysis and the Krebs-cycle via increased gene expression and activity of AIaAT allied to succinate accumulation in roots of Fundacep. This may represents a metabolic advantage for this genotype over Macota with regard to hypoxia tolerance. (C) 2016 Elsevier B.V. All rights reserved.
Subject: Glycine Max
Waterlogging
Amino Acids
Organic Acids
Alanine Aminotransferase
Editor: Pergamon-Elsevier Science LTD
Oxford
Rights: fechado
Identifier DOI: 10.1016/j.envexpbot.2016.10.007
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0098847216302180
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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