Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326510
Type: Artigo
Title: Hsp70 Of Leishmania Amazonensis Alters Resistance To Different Stresses And Mitochondrial Bioenergetics
Author: Codonho
Barbara Santoni; Costa
Solange dos Santos; Peloso
Eduardo de Figueiredo; Joazeiro
Paulo Pinto; Gadelha
Fernanda Ramos; Giorgio
Selma
Abstract: The 70 kDa heat shock protein (HSP70) is a molecular chaperone that assists the parasite Leishmania in returning to homeostasis after being subjected to different types of stress during its life cycle. In the present study, we evaluated the effects of HSP70 transfection of L. amazonensis promastigotes (pTEX-HSP70) in terms of morphology, resistance, infectivity and mitochondrial bioenergetics. The pTEX-HSP70 promastigotes showed no ultrastructural morphological changes compared to control parasites. Interestingly, the pTEX-HSP70 promastigotes are resistant to heat shock, H2O2-induced oxidative stress and hyperbaric environments. Regarding the bioenergetics parameters, the pTEX-HSP70 parasites had higher respiratory rates and released less H2O2 than the control parasites. Nevertheless, the infectivity capacity of the parasites did not change, as verified by the infection of murine peritoneal macrophages and human macrophages, as well as the infection of BALB/c mice. Together, these results indicate that the overexpression of HSP70 protects L. amazonensis from stress, but does not interfere with its infective capacity.
Subject: Leishmania Amazonensis
Hsp70
Mitochondria
Oxidative Stress
Hyperbaric Oxygen
Editor: Fundação Oswaldo Cruz, Escola Politécnica Saúde Joaquim Venâncio
Rio de Janeiro
Rights: aberto
Identifier DOI: 10.1590/0074-02760160087
Address: http://www.scielo.br/scielo.php?pid=S0074-02762016000700460&script=sci_arttext
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
000380112500007.pdf1.24 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.