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Type: Artigo
Title: Hsp70 Of Leishmania Amazonensis Alters Resistance To Different Stresses And Mitochondrial Bioenergetics
Author: Codonho
Barbara Santoni; Costa
Solange dos Santos; Peloso
Eduardo de Figueiredo; Joazeiro
Paulo Pinto; Gadelha
Fernanda Ramos; Giorgio
Abstract: The 70 kDa heat shock protein (HSP70) is a molecular chaperone that assists the parasite Leishmania in returning to homeostasis after being subjected to different types of stress during its life cycle. In the present study, we evaluated the effects of HSP70 transfection of L. amazonensis promastigotes (pTEX-HSP70) in terms of morphology, resistance, infectivity and mitochondrial bioenergetics. The pTEX-HSP70 promastigotes showed no ultrastructural morphological changes compared to control parasites. Interestingly, the pTEX-HSP70 promastigotes are resistant to heat shock, H2O2-induced oxidative stress and hyperbaric environments. Regarding the bioenergetics parameters, the pTEX-HSP70 parasites had higher respiratory rates and released less H2O2 than the control parasites. Nevertheless, the infectivity capacity of the parasites did not change, as verified by the infection of murine peritoneal macrophages and human macrophages, as well as the infection of BALB/c mice. Together, these results indicate that the overexpression of HSP70 protects L. amazonensis from stress, but does not interfere with its infective capacity.
Subject: Leishmania Amazonensis
Oxidative Stress
Hyperbaric Oxygen
Editor: Fundação Oswaldo Cruz, Escola Politécnica Saúde Joaquim Venâncio
Rio de Janeiro
Citation: Memorias Do Instituto Oswaldo Cruz. Fundação Oswaldo Cruz, Escola Politécnica Saúde Joaquim Venâncio, v. 111, p. 460 - 468, 2016.
Rights: aberto
Identifier DOI: 10.1590/0074-02760160087
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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