Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326496
Type: Artigo
Title: Initial Oral Biofilm Formation On Titanium Implants With Different Surface Treatments: An In Vivo Study
Author: Ribeiro
Cyntia Ferreira; Cogo-Muller
Karina; Franco
Gilson Cesar; Silva-Concilio
Lais Regiane; Campos
Marcia Sampaio; Rode
Sigmar de Mello; Claro Neves
Ana Christina
Abstract: The aim of this study was to examine in vivo the initial bacterial adhesion on titanium implants with different surface treatments. Design: Ten subjects wore oral splints containing machined pure titanium disks (Ti-M), acid-etched titanium (Ti-AE) and anodized and laser irradiated disks (Ti-AL) for 24 h. After this period, disks were removed from the splints and adherent bacteria were quantified by an enzymatic assay to assess total viable bacteria and by Real Time PCR to evaluate total bacteria and Streptococcus oralis levels. Additionally, the initial adherent microorganisms were visualized by scanning electron microscopy (SEM). Titanium surface morphology was verified using SEM, and roughness was evaluated by profilometer analysis. Results: Regarding titanium surface roughness, Ti-AL (1.423 +/- 0.397) showed significantly higher Ra values than did Ti-M (0.771 +/- 0.182) and Ti-AE (0.735 +/- 0.196) (p < 0.05, ANOVA - Tahame). Ti-AE and Ti-AL presented roughened micro-structure surfaces characterized by open pores, whereas Ti-M showed long grooves alternating with planed areas. Comparing the Ti-M, Ti-AE and Ti-AL groups for viable bacteria (MIT assay), total bacteria and S. oralis quantification (qPCR), no significant differences were observed among these three groups (p > 0.05, ANOVA - Tahame). SEM images showed similar bacterial adhesion on the three titanium surfaces, predominantly characterized by cocci and several bacilli, indicating an initial colonization of the oral biofilm. Conclusion: In conclusion, roughness and microtopography did not stimulate initial biofilm formation on titanium surfaces with different surface treatments. (C) 2016 Elsevier Ltd. All rights reserved.
Subject: Biofilm
Titanium
Implants
Surface Properties
Streptococcus Oralis
Editor: Pergamon-Elsevier Science LTD
Oxford
Rights: fechado
Identifier DOI: 10.1016/j.archoralbio.2016.05.006
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0003996916301169
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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