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Type: Congresso
Title: Enzymatic Profiling In Prostate And Breast Cancer Cells: Phosphate Hydrolysis And Alcohol Oxidation
Author: Goncalves
Caroline C. S.; da Costa
Bruna Z.; Lima
Maria L. S. O.; Fiorito
Giovanna F.; Ruiz
Ana Lucia T. G.; de Oliveira
Silvia B. P.; Barbosa
Guilherme O.; de Carvalho
Hernandes F.; Marsaioli
Anita Jocelyne
Abstract: Enzyme activities in non-tumor and cancer cells, in different cell compartments, were visualized with two small fluorogenic probes: 7-(3-hydroxypropy1)-resorufin (7) and 7-(3-phosphatepropyl)-resorufin (9), with a primary alcohol and a phosphate group as enzymatic recognition sites. The designed probes were evaluated by in vitro assays, proving that both were biotransformed by their respective enzymes (probe 7 by alcohol dehydrogenases, ADHs, and probe 9 by a protein serine/threonine phosphatase, PSTP). Additionally, joint action of both enzymes (ADH and PSTP) revealed that probe 9 underwent a multi-enzymatic cascade and that phosphate hydrolysis is the rate-limiting step. These probes were evaluated in four human cell lines: prostate cancer cells (PC-3), prostate cells (RWPE-1), breast cancer cells (MCF-7 BUS) and breast cells (MCF-10A). Flow cytometry experiments showed distinct profiles for prostate and breast cells and assays with phosphatase inhibitors confirmed the proposed multi enzymatic cascade in living cells. (C) 2016 Published by Elsevier Ltd.
Subject: Alcohol Dehydrogenase
Prostate Cancer Cells
Breast Cancer Cells
Editor: Pergamon-Elsevier Science LTD
Rights: fechado
Identifier DOI: 10.1016/j.tet.2015.11.061
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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