Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326408
Type: Artigo
Title: Low Sequence Identity But High Structural And Functional Conservation: The Case Of Hsp70/hsp90 Organizing Protein (hop/sti1) Of Leishmania Braziliensis
Author: Batista
Fernanda A. H.; Seraphim
Thiago V.; Santos
Clelton A.; Gonzaga
Marisvanda R.; Barbosa
Leandro R. S.; Ramos
Carlos H. I.; Borges
Julio C.
Abstract: Parasites belonging to the genus Leishmania are subjected to extensive environmental changes during their life cycle; molecular chaperones/co-chaperones act as protagonists in this scenario to maintain cellular homeostasis. Hop/Sti1 is a co-chaperone that connects the Hsp90 and Hsp70 systems, modulating their ATPase activities and affecting the fate of client proteins because it facilitates their transfer from the Hsp70 to the Hsp90 chaperone. Hop/Sti1 is one of the most prevalent co-chaperones, highlighting its importance despite the relatively low sequence identity among orthologue proteins. This multi-domain protein comprises three tetratricopeptides domains (TPR1, TPR2A and TPR2B) and two Asp/Pro-rich domains. Given the importance of Hop/Sti1 for the chaperone system and for Leishmania protozoa viability, the Leishmania braziliensis Hop (LbHop) and a truncated mutant (LbHop(TPR2AB)) were characterized. Structurally, both proteins are alpha-helix-rich and highly elongated monomeric proteins. Functionally, they inhibited the ATPase activity of Leishmania braziliensis Hsp90 (LbHsp90) to a similar extent, and the thermodynamic parameters of their interactions with LbHsp90 were similar, indicating that TPR2A-TPR2B forms the functional center for the LbHop interaction with LbHsp90. These results highlight the structural and functional similarity of Hop/Sti1 proteins, despite their low sequence conservation compared to the Hsp70 and Hsp90 systems, which are phylogenetic highly conserved. (C) 2016 Elsevier Inc. All rights reserved.
Subject: Hop
Hsp90
Tpr
Molecular Chaperones
L. Braziliensis
Protozoa
Editor: Elsevier Science INC
New York
Rights: fechado
Identifier DOI: 10.1016/j.abb.2016.04.008
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S000398611630128X
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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