Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326407
Type: Artigo
Title: Application Of A Nanostructured Platform And Imprinted Sol-gel Film For Determination Of Chlorogenic Acid In Food Samples
Author: Ribeiro
Carla M.; Miguel
Eliane M.; Silva
Jonadab dos S.; da Silva
Cristian B.; Goulart
Marilia O. F.; Kubota
Lauro T.; Gonzaga
Fabiano B.; Santos
Wilney J. R.; Lima
Phabyanno R.
Abstract: Chlorogenic acid (CGA) is a polyphenol derivative that widely exists in higher plants like fruits, vegetables, black teas, and some traditional Chinese medicines. In this work, we have proposed a sensitive and selective electrochemical sensor for detection of CGA. The sensor was based on a glassy carbon electrode (GCE) modified with a functional platform by grafting vinyltrimethoxysilane (VTMS) in multi walled carbon nanotubes (MWCNTs) and covered by a molecularly imprinted siloxane (MIS) film prepared using the sol-gel process. The VTMS was grafted onto the surface of the MWCNTs via in situ free radical polymerization. The MIS was obtained from the acid-catalyzed hydrolysis/condensation of a solution consisting of tetraethoxysilane (TEOS), phenyltriethoxysilane (PTEOS), (3-aminopropyl)tri-methoxysilane (APTMS), and CGA as a template molecule. The modification procedure was evaluated by differential pulse voltammetry (DPV) and scanning electron microscopy (SEM). Under optimized operational conditions, a linear response was obtained covering a concentration ranging from 0.08 mu mol L-1 to 500 mu mol L-1 with a detection limit (LOD) of 0.032 mu mol L-1. The proposed sensor was applied to CGA determination in coffee, tomato, and apple samples with recoveries ranging from 99.3% to 108.6%, showing a promising potential application in food samples. Additionally, the imprinted sensor showed a significantly higher affinity for target CGA than the non-imprinted siloxane (NIS) sensor. (C) 2016 Elsevier B.V. All rights reserved.
Subject: Chlorogenic Acid
Molecular Imprinting Siloxane
Sol-gel
Mwcnts
Editor: Elsevier Science BV
Amsterdam
Rights: fechado
Identifier DOI: 10.1016/j.talanta.2016.05.020
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0039914016303381
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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