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Type: Artigo
Title: Host Transcriptional Profiling At Early And Later Stages Of The Compatible Interaction Between Phaseolus Vulgaris And Meloidogyne Incognita
Author: Santini
Luciane; Munhoz
Carla de Freitas; Bonfim
Mauro Ferreira
Jr.; Brandao
Marcelo Mendes; Inomoto
Mario Massayuki; Carneiro Vieira
Maria Lucia
Abstract: The root-knot nematode (Meloidogyne incognita), is one of most devastating pathogens that attack the common bean crop. Although there is evidence that some cultivars have race-specific resistance against M. incognita, these resistance sources have not proved effective, and nematodes are able to circumvent the host's defense system. We constructed RNA-seq based libraries and used a high-throughput sequencing platform to analyze the plant responses to M. incognita. Assessments were performed at 4 and 10 days after inoculation corresponding. to the stages of nematode penetration and giant cell development, respectively. Large-scale transcript mapping to, the common bean reference genome (G19833) resulted in the identification of 27,195 unigenes. Of these, 797 host genes were found to be differentially expressed. The functional annotation results confirm the complex interplay between abiotic and biotic stress signaling pathways. High expression levels of the wounding-responsive genes were observed over the interaction. At early response, an overexpression of the N gene, a TIR-NBS-LRR resistance gene, was understood as a host attempt to overcome the pathogen attack. However, the repression of heat shock proteins resulted in a lack of reactive oxygen species accumulation and absence of a hypersensitive response. Furthermore, the host basal response was broken by the repression of the ethylene/jasmonate pathway later in the response, resulting in a continuous compatible process with consequent plant susceptibility.
Subject: Plant Pathogen Interaction
Rna Sequencing
Editor: Amer Phytopathological Soc
St Paul
Citation: Phytopathology. Amer Phytopathological Soc , v. 106, p. 282 - 294, 2016.
Rights: fechado
Identifier DOI: 10.1094/PHYTO-07-15-0160-R
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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