Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326266
Type: Artigo
Title: Bioaugmentation Strategy Employing A Microbial Consortium Immobilized In Chitosan Beads For Oil Degradation In Mesocosm Scale
Author: Dellagnezze
B. M.; Vasconcellos
S. P.; Angelim
A. L.; Melo
V. M. M.; Santisi
S.; Cappello
S.; Oliveira
V. M.
Abstract: A bacterial consortium composed by four metagenomic clones and Bacillus subtilis strain CBMAI 707, all derived froth petroleum reservoirs, was entrapped in chitosan beads and evaluated regarding hydrocarbon degradation capability. Experiments were carried out in mesocosm scale (3000 L) with seawater artificially polluted with crude oil.At different time intervals, mesocosms were sampled and subjected to GC-FID and microbiological analyses, as total and heterotrophic culturable bacterial abundance (DAPI and CFLI count), biological oxygen demand (BOD) and taxonomic diversity (massive sequencing of 16S rRNA genes). The results obtained showed that degradation of n-alkane hydrocarbons was similar between both treatments. However, aromatic compound degradation was more efficient in bioaugmentation treatment, with biodegradation percentages reaching up to 99% in 30 days. Community dynamics was different between treatments and the consortium used in the bioaugmentation treatment contributed to a significant increase in aromatic hydrocarbon degradation. (C) 2016 Elsevier Ltd. All rights reserved.
Subject: Bioaugmentation
Mesocosm
Metagenomic Clones
Consortium
Petroleum
Editor: Pergamon-Elsevier Science LTD
Oxford
Rights: fechado
Identifier DOI: 10.1016/j.marpolbul.2016.04.011
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0025326X16302120?via%3Dihub
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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