Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326245
Type: Artigo
Title: Limonene Reduces Hyperalgesia Induced By Gp120 And Cytokines By Modulation Of Il-1 Beta And Protein Expression In Spinal Cord Of Mice
Author: Piccinelli
Ana Claudia; Morato
Priscila Neder; Barbosa
Marcelo dos Santos; Croda
Julio; Sampson
Jared; Kong
Xiangpeng; Konkiewitz
Elisabete Castelon; Ziff
Edward B.; Amaya-Farfan
Jaime; Leite Kassuya
Cndida Aparecida
Abstract: We have investigated the antihyperalgesic effects of limonene in mice that received intrathecal injection of gp120. Main methods: Male Swiss mice received gp120, IL-1 beta or TNF-alpha intrathecally or sterile saline as a control. A mechanical sensitivity test was performed at 2 and 3 h after the injection. Spinal cord and blood samples were isolated for protein quantification. Key findings: Intrathecal administration of gp120 increased mechanical sensitivity measured with an electronic Von Frey apparatus, at 2 and 3 h after the injections. Limonene administered orally prior to gp120 administration significantly decreased this mechanical sensitivity at 3 h after the gp120 injection. In addition, intrathecal injection of gp120 increased IL-1 beta and IL-10 in serum, and limonene prevented the ability of gp120 to increase these cytokines. Limonene also inhibited TNF-alpha and IL-1 beta-induced mechanical hyperalgesia. Western blot assay demonstrated limonene was capable of increasing SOD expression in the cytoplasm of cells from spinal cord at 4 h after intrathecal IL-1 beta injection. Significance: These results demonstrate that gp120 causes mechanical hyperalgesia and a peripheral increase in IL-1 beta and IL-10, and that prior administration of limonene inhibits these changes. Also limonene modulates the activation of SOD expression in the spinal cord after spinal IL-1 beta application. The ability of limonene to inhibit the mechanical hyperalgesia induced by gp120, TNF-alpha and IL-1 beta emphasizes the anti-inflammatory action of limonene, specifically its ability to inhibit cytokine production and its consequences. (C) 2016 Published by Elsevier Inc.
Subject: Mechanical Hyperalgesia
Gp120
Il-1 Beta
Tnf-alpha
Editor: Pergamon-Elsevier Science LTD
Oxford
Rights: fechado
Identifier DOI: 10.1016/j.lfs.2016.11.017
Address: http://www-sciencedirect-com.ez88.periodicos.capes.gov.br/science/article/pii/S0024320516306695
Date Issue: 2017
Appears in Collections:Unicamp - Artigos e Outros Documentos

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