Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/326243
Type: Artigo
Title: Amperometric Quantification Of S-nitrosoglutathione Using Gold Nanoparticles: A Step Toward Determination Of S-nitrosothiols In Plasma
Author: Baldim
Victor; Ismail
Abdulghani; Taladriz-Blanco
Patricia; Griveau
Sophie; de Oliveira
Marcelo Ganzarolli; Bedioui
Fethi
Abstract: S-Nitrosothiols (RSNOs) are carriers of nitric oxide (NO) and have important biological activities. We propose here the use of gold nanoparticles (AuNPs) and NO-selective amperometric microsensor for the detection and quantification Of S-nitro-soglutathione (GSNO) as a step toward the determination of plasma RSNOs. AuNPs were used to decompose RSNOs with the quantitative release of free NO which was selectively detected, with a NO microsensor. The optimal [GSNO]/[AuNPs] ratio was determined, corresponding to an excess of AuNP surface relative to the molar GSNO amount. Moreover, the influence of free plasma thiols on this method was investigated and a protocol based on the blocking of free thiols, with iodoacetic acid, forming the carboxymethyl derivative of the cysteine residues, is proposed.
Editor: Amer Chemical Soc
Washington
Rights: fechado
Identifier DOI: 10.1021/acs.analchem.5b04035
Address: http://pubs-acs-org.ez88.periodicos.capes.gov.br/doi/abs/10.1021/acs.analchem.5b04035
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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