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Type: Artigo
Title: Oral Cavities Of Healthy Infants Harbour High Proportions Of Streptococcus Salivarius Strains With Phenotypic And Genotypic Resistance To Multiple Classes Of Antibiotics
Author: Palma
Thais H.; Harth-Chu
Erika N.; Scott
Jodie; Stipp
Rafael N.; Boisvert
Heike; Salomao
Mariana F.; Theobaldo
Jessica D.; Possobon
Rosana F.; Nascimento
Leandro C.; McCafferty
Jonathan W.; Faller
Lina; Duncan
Margaret J.; Mattos-Graner
Renata O.
Abstract: Emerging antibiotic resistance in the oropharyngeal microbiota, of which Streptococcus salivarius is a prominent species, represents a challenge for treating paediatric populations. In this study, we investigated the role of Streptococcus salivarius as a reservoir for antibiotic resistance genes (ARG) in the oral microbiota by analysing 95 Streptococcus salivarius isolates from 22 healthy infants (2-16 months of age). MICs of penicillin G, amoxicillin, erythromycin, tetracycline, doxycycline and streptomycin were determined. ARG profiles were assessed in a subset of 21 strains by next-generation sequencing of genomes, followed by searches of assembled reads against the Comprehensive Antibiotic Resistance Database. Strains resistant to erythromycin, penicillins and tetracyclines were isolated from 83.3, 33.3 and 16.6 %, respectively, of infants aged 2 to 8 months with no prior antibiotic treatment. These percentages were 100.0, 66.6 and 50.0 %, by 13 to 16 months of age. ARG or polymorphisms associated with antibiotic resistance were the most prevalent and involved genes for macrolide efflux (mel, mefA/E and macB), ribosomal protection [erm(B), tet(M) and tet(O)] and beta-lactamase-like proteins. Phylogenetically related strains showing multidrug-resistant phenotypes harboured multidrug efflux ARG. Polymorphic genes associated with antibiotic resistance to drugs affecting DNA replication, folate synthesis, RNA/protein synthesis and regulators of antibiotic stress responses were detected. These data imply that Streptococcus salivarius strains established during maturation of the oral microbiota harbour a diverse array of functional ARG, even in the absence of antibiotic selective pressures, highlighting a potential role for this species in shaping antibiotic susceptibility profiles of oropharyngeal communities.
Editor: Microbiology Soc
Citation: Journal Of Medical Microbiology. Microbiology Soc, v. 65, p. 1456 - 1464, 2016.
Rights: fechado
Identifier DOI: 10.1099/jmm.0.000377
Date Issue: 2016
Appears in Collections:Unicamp - Artigos e Outros Documentos

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